Overview

  • Product nameAnti-Lyn antibody [Y497]
    See all Lyn primary antibodies
  • Description
    Rabbit monoclonal [Y497] to Lyn
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues close to SH3 domain of human Lyn

  • EpitopeResidues close to SH3 domain of human Lyn
  • Positive control
    • Hela cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberY497
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 53 kDa).
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC.
  • Target

    • FunctionDown regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
    • Tissue specificityWidely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • DomainThe protein kinase domain plays an important role in its localization in the cell membrane.
    • Post-translational
      modifications
      Ubiquitinated. Ubiquitination is SH3-dependent.
    • Cellular localizationCell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
    • Information by UniProt
    • Database links
    • FormThis protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • Alternative names
      • AA407514 antibody
      • EC 2.7.10.2 antibody
      • FLJ26625 antibody
      • Hck 2 antibody
      • JTK 8 antibody
      • JTK8 antibody
      • Lck/Yes related novel protein tyrosine kinase antibody
      • LYN antibody
      • LYN proto oncogene, Src family tyrosine kinase antibody
      • LYN_HUMAN antibody
      • ONCOGENE LYN antibody
      • p53Lyn antibody
      • p56Lyn antibody
      • Tyrosine protein kinase LYN antibody
      • Tyrosine-protein kinase Lyn antibody
      • V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
      • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
      see all

    Anti-Lyn antibody [Y497] images



    • Predicted band size : 53 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Lyn knockout HAP1 cell lysate (20 µg)
      Lane 3: Ramos cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab32398 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32398 was shown to specifically react with Lyn when Lyn knockout samples were used. Wild-type and Lyn knockout samples were subjected to SDS-PAGE. ab32398 and ab8245 (loading control to GAPDH) were diluted at 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Lyn antibody [Y497] (ab32398) at 1/2000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : Rat liver tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 53 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)

      Lanes 1 - 3: Merged signal (red and green). Green - ab32398 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa. 

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32398 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

    • Anti-Lyn antibody [Y497] (ab32398) at 1/2000 dilution + Hela cell lysate

      Predicted band size : 53 kDa
      Observed band size : 56 kDa (why is the actual band size different from the predicted?)

    References for Anti-Lyn antibody [Y497] (ab32398)

    This product has been referenced in:
    • Lee EJ  et al. Capsiate inhibits ultraviolet B-induced skin inflammation by inhibiting Src family kinases and epidermal growth factor receptor signaling. Free Radic Biol Med 48:1133-43 (2010). Read more (PubMed: 20123015) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. I can confirm that ab32398 Lyn antibody should detect both versions of Lyn. I hope this information willbe useful for you.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"