ab53122 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/300 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
Use at an assay dependent concentration.
Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.
The protein kinase domain plays an important role in its localization in the cell membrane.
Ubiquitinated. Ubiquitination is SH3-dependent.
Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
EC 188.8.131.52 antibody
Hck 2 antibody
JTK 8 antibody
Lck/Yes related novel protein tyrosine kinase antibody
LYN proto oncogene, Src family tyrosine kinase antibody
ONCOGENE LYN antibody
Tyrosine protein kinase LYN antibody
Tyrosine-protein kinase Lyn antibody
V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
Western blot - Lyn (phospho Y507) antibody (ab53122)
All lanes : Anti-Lyn (phospho Y507) antibody (ab53122) at 1/300 dilution
Lane 1 : K562 cell extract treated with Hydrogen Peroxide (100uM, 30mins) Lane 2 : K562 cell extract treated with Hydrogen Peroxide (100uM, 30mins) with immunising peptide