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A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 507 of Lyn (Human).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab33914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/500 - 1/5000. Detects a band of approximately 53-56 kDa (predicted molecular weight: 61 kDa).|
Blocking and dilution buffer: 5% NFDM/TBST.
Dot blot analysis of Lyn (phospho Y507) phospho peptide (Lane 1) and Lyn non-phospho peptide (Lane 2) labelling Lyn (phospho Y507) with ab33914 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.
Blocking and dilution buffer: 5% NFDM /TBST.