Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)

Overview

  • Product name
    Anti-Lyn (phospho Y507) antibody [EP504Y]
    See all Lyn primary antibodies
  • Description
    Rabbit monoclonal [EP504Y] to Lyn (phospho Y507)
  • Specificity
    The antibody will detect Lyn’s phosphorylation on Tyrosine 507.
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 507 of Lyn (Human).

  • Positive control
    • WB: HeLa (untreated and treated with pervanadate) and Raji treated with pervanadate whole cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP504Y
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab33914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/5000. Detects a band of approximately 53-56 kDa (predicted molecular weight: 61 kDa).
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
    • Tissue specificity
      Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Domain
      The protein kinase domain plays an important role in its localization in the cell membrane.
    • Post-translational
      modifications
      Ubiquitinated. Ubiquitination is SH3-dependent.
    • Cellular localization
      Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
    • Information by UniProt
    • Database links
    • Form
      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • Alternative names
      • AA407514 antibody
      • EC 2.7.10.2 antibody
      • FLJ26625 antibody
      • Hck 2 antibody
      • JTK 8 antibody
      • JTK8 antibody
      • Lck/Yes related novel protein tyrosine kinase antibody
      • LYN antibody
      • LYN proto oncogene, Src family tyrosine kinase antibody
      • LYN_HUMAN antibody
      • ONCOGENE LYN antibody
      • p53Lyn antibody
      • p56Lyn antibody
      • Tyrosine protein kinase LYN antibody
      • Tyrosine-protein kinase Lyn antibody
      • V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
      • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
      see all

    Images

    • All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/1000 dilution

      Lane 1 : Raji whole cell lysate
      Lane 2 : Raji treated with 1mM pervanadate for 30 min whole cell lysate
      Lane 3 : Raji treated with 1mM pervanadate for 30 min whole cell lysate, the membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 61 kDa
      Observed band size : 61 kDa

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of Lyn (phospho Y507) phospho peptide (Lane 1) and Lyn non-phospho peptide (Lane 2) labelling Lyn (phospho Y507) with ab33914 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.

      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/5000 dilution

      Lane 1 : 10ug Non treated HeLa cell lysate
      Lane 2 : 10ug HeLa cell lysate treated with Pervanadate

      Secondary
      Goat anti-rabbit HRP labeled.

      Predicted band size : 61 kDa

    References

    This product has been referenced in:
    • Sharma N  et al. SHP2 phosphatase promotes mast cell chemotaxis toward stem cell factor via enhancing activation of the Lyn/Vav/Rac signaling axis. J Immunol 192:4859-66 (2014). Read more (PubMed: 24733849) »
    • Luo W  et al. A balance between B cell receptor and inhibitory receptor signaling controls plasma cell differentiation by maintaining optimal Ets1 levels. J Immunol 193:909-20 (2014). Read more (PubMed: 24929000) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry.

    These 2 antibodies have lot specific concentrations. If you could please forward me the lot numbers for ab40660 and ab33914 I'd be happy to enquire about the concentrations.

    Thanks for the additional info...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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