Recombinant Anti-LYPD3 antibody [SP204] (ab183353)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP204] to LYPD3
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LYPD3 antibody [SP204]
See all LYPD3 primary antibodies -
Description
Rabbit monoclonal [SP204] to LYPD3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human skin, esophagus, cervix, tonsil, skin squamous cell carcinoma, and cervical squamous cell carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP204 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression. -
Tissue specificity
Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers. -
Sequence similarities
Contains 2 UPAR/Ly6 domains. -
Post-translational
modificationsN-glycosylated and O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 27076 Human
- Omim: 609484 Human
- SwissProt: O95274 Human
- Unigene: 631594 Human
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Alternative names
- 2310061G07Rik antibody
- C4.4A antibody
- GPI-anchored metastasis-associated protein C4.4A homolog antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervix carcinoma tissue sections labeling LYPD3 with ab183353 at 1/100 dilution (3.95 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling LYPD3 with ab183353 at 1/100 dilution (3.95 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemical analysis of paraffin embedded Human skin tissue labeling LYPD3 with ab183353 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded Human esophagus tissue labeling LYPD3 with ab183353 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded Human cervix tissue labeling LYPD3 with ab183353 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling LYPD3 with ab183353 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded Human skin squamous cell carcinoma tissue labeling LYPD3 with ab183353 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded Human cervical squamous cell carcinoma tissue labeling LYPD3 with ab183353 at 1/100 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183353 has not yet been referenced specifically in any publications.