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Rabbit polyclonal to LYPLA3
Predicted to work with:
Mouse, Rat, Cow, Dog
Recombinant fragment, corresponding to a region within amino acids 1-177 of Human LYPLA3 (AAH62605).
293T, A431 and Jurkat cells and whole cell lysates; Human Colon carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
ab111898 is purified by antigen-affinity chromatography.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) may alternatively be used for heat-mediated antigen retrieval.
1/100 - 1/500.
Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (By similarity). May have weak lysophospholipase activity.
Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Belongs to the AB hydrolase superfamily. Lipase family.
Information by UniProt
1 O acylceramide synthase antibody
1-O-acylceramide synthase antibody
Western blot - LYPLA3 antibody (ab111898)
Anti-LYPLA3 antibody (ab111898) at 1/2000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 47 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - LYPLA3 antibody (ab111898)
ab111898 at 1/200 dilution staining LYPLA3 in methanol-fixed A431 cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LYPLA3 antibody (ab111898)
ab111898 at 1/500 dilution staining LYPLA3 in Formalin-fixed, Paraffin-embedded Human colon carcinoma by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"