Overview

  • Product name
  • Description
    Rabbit polyclonal to LYPLA3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-177 of Human LYPLA3 (AAH62605).

  • Positive control
    • 293T, A431 and Jurkat cells and whole cell lysates; Human Colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) may alternatively be used for heat-mediated antigen retrieval.
ICC/IF 1/100 - 1/500.

Target

  • Function
    Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (By similarity). May have weak lysophospholipase activity.
  • Tissue specificity
    Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the AB hydrolase superfamily. Lipase family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Lysosome. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 O acylceramide synthase antibody
    • 1-O-acylceramide synthase antibody
    • ACS antibody
    • DKFZp564A0122 antibody
    • Group XV phospholipase A2 antibody
    • GXVPLA 2 antibody
    • GXVPLA2 antibody
    • LCAT like lysophospholipase antibody
    • LCAT-like lysophospholipase antibody
    • LLPL antibody
    • LPLA 2 antibody
    • LPLA2 antibody
    • LYPLA 3 antibody
    • Lysophospholipase 3 (lysosomal phospholipase A2) antibody
    • Lysophospholipase 3 antibody
    • Lysosomal phospholipase A2 antibody
    • OTTHUMP00000174859 antibody
    • PAG15_HUMAN antibody
    • Phospholipase A2, group XV antibody
    • PLA2G15 antibody
    • UNQ341/PRO540 antibody
    see all

Images

  • Anti-LYPLA3 antibody (ab111898) at 1/2000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size : 47 kDa
  • ab111898 at 1/200 dilution staining LYPLA3 in methanol-fixed A431 cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
  • ab111898 at 1/500 dilution staining LYPLA3 in Formalin-fixed, Paraffin-embedded Human colon carcinoma by Immunohistochemistry.

References

ab111898 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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