Overview

  • Product name
  • Description
    Rabbit polyclonal to LYPLA3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 224-412 of Human LYPLA3 (UniProt ID: Q8NCC3).

  • Positive control
    • HepG2 cell lysate, Human colon carcinoma tissue.
  • General notes
    Store as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval buffer: Tris-EDTA buffer pH 8.

Target

  • Function
    Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (By similarity). May have weak lysophospholipase activity.
  • Tissue specificity
    Highly expressed in heart, placenta, skeletal muscle, kidney and pancreas. Detected at lower levels in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the AB hydrolase superfamily. Lipase family.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Lysosome. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 O acylceramide synthase antibody
    • 1-O-acylceramide synthase antibody
    • ACS antibody
    • DKFZp564A0122 antibody
    • Group XV phospholipase A2 antibody
    • GXVPLA 2 antibody
    • GXVPLA2 antibody
    • LCAT like lysophospholipase antibody
    • LCAT-like lysophospholipase antibody
    • LLPL antibody
    • LPLA 2 antibody
    • LPLA2 antibody
    • LYPLA 3 antibody
    • Lysophospholipase 3 (lysosomal phospholipase A2) antibody
    • Lysophospholipase 3 antibody
    • Lysosomal phospholipase A2 antibody
    • OTTHUMP00000174859 antibody
    • PAG15_HUMAN antibody
    • Phospholipase A2, group XV antibody
    • PLA2G15 antibody
    • UNQ341/PRO540 antibody
    see all

Images

  • Anti-LYPLA3 antibody (ab154012) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue, labeling LYPLA3 with ab154012 at 1/500 dilution.

References

ab154012 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154012.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up