Anti-Lysophospholipase 1 antibody [EPR3666] (ab91600)


  • Product nameAnti-Lysophospholipase 1 antibody [EPR3666]
    See all Lysophospholipase 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3666] to Lysophospholipase 1
  • Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Lysophospholipase 1.

  • Positive control
    • Human fetal liver, Human fetal heart and Human spleen cell lysates.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR3666
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab91600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 21, 24 kDa (predicted molecular weight: 25 kDa).
ICC 1/500 - 1/1000.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    Anti-Lysophospholipase 1 antibody [EPR3666] images

    • Predicted band size : 25 kDa

      Lane 1: Wild type HAP1 whole cell lysate (40 µg)
      Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)
      Lane 3: Mouse spleen whole cell lysate (40 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab91600 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab91600 was shown to specifically react with LYPLA1 when LYPLA1 knockout samples were used. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE. ab91600 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with ab216773, Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed and ab216776, Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Lysophospholipase 1 antibody [EPR3666] (ab91600) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 25 kDa

    References for Anti-Lysophospholipase 1 antibody [EPR3666] (ab91600)

    ab91600 has not yet been referenced specifically in any publications.

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