Lysosomal Staining Kit - Blue Fluorescence - Cytopainter (ab112135)

Overview

  • Product name
    Lysosomal Staining Kit - Blue Fluorescence - Cytopainter
    See all Lysosome kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Product overview

    Lysosomal Staining Kit - Blue Fluorescence | Cytopainter (ab112135) is designed to label lysosomes in live cells in blue fluorescence at Ex/Em = 350/440nm. Ab112135 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background. The labeling protocol is robust, requiring minimal hands-on time.


    Ab112135 can be readily adapted for many different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. Ab112135 provides all the essential components with an optimized cell-labeling protocol and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

  • Notes

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria,and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

  • Tested applications
    Suitable for: FM, ICC/IFmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab112135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • ab112135 staining U2OS cells by Immunofluorescence (in a black 96 well plate).

Protocols

References

This product has been referenced in:
  • Liu F  et al. Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo. Int J Nanomedicine 12:5135-5148 (2017). Read more (PubMed: 28790821) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you very much for your inquiry. I am sorry to hear you have been experiencing problems with the CytoPainter Lysosomal Staining Kit. Due to the wide emission spectrum property of the CytoPainter dye it is normal to also have some green fluo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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