Lysosomal Staining Kit - Green Fluorescence - Cytopainter (ab112136)

Overview

  • Product name
    Lysosomal Staining Kit - Green Fluorescence - Cytopainter
    See all Lysosome kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based
  • Product overview

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    ab112136 is designed to label lysosomes in live cells with green fluorescence at Ex/Em = 490/525 nm. The proprietary lysotropic dye used in the kit selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

  • Tested applications
    Suitable for: FMmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab112136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.

Images

  • ab112136 staining U2OS cells by fluorescence microscopy (in a black 96 well plate).

Protocols

References

This product has been referenced in:
  • Liu F  et al. Parallel comparative studies on toxicity of quantum dots synthesized and surface engineered with different methods in vitro and in vivo. Int J Nanomedicine 12:5135-5148 (2017). Read more (PubMed: 28790821) »
  • Aasen SN  et al. A Novel Nanoprobe for Multimodal Imaging Is Effectively Incorporated into Human Melanoma Metastatic Cell Lines. Int J Mol Sci 16:21658-80 (2015). Read more (PubMed: 26370983) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.





They are mostly in lysosomes, but also may go to acidic organelles.


The green fluorescence of the ab112136 lysosome stain does not persist after PFA fixation, but the fluorescence of CytoPainter lysosome stains that contain these other fluorochromes does:

Orange - ab138895

Red - ab112137
Read More

Vielen Dank für Ihren Anruf heute morgen.

Ich haben nun unserer Katalog etwas besser durchgeschaut, und möchte mal dieVorschläge hier unten machen.

Als ich nach Zielproteinen geschaut habe, ging ich so vor: Es hat Lys...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up