For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Lysozyme isolated from urine of patients with monocytic leukemia.
Our Abpromise guarantee covers the use of ab2408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||1/50 - 1/100.|
|IHC-Fr||Use at an assay dependent concentration.|
Lysozyme was immunoprecipitated using 0.5mg HL60 whole cell extract, 5µg of Rabbit polyclonal to Lysozyme and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HL60 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2408.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 16kDa; Lysozyme
ab2408 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"