Anti-Lysozyme antibody (ab2408)

Overview

  • Product nameAnti-Lysozyme antibodySee all Lysozyme primary antibodies ...
  • Description
    Rabbit polyclonal to Lysozyme
  • SpecificityThis antibody reacts with lysozyme. It stains granulocytes, monocytes and macrophages in human tonsil, skin and colon. This antibody does not cross-react with any other cell types.
  • Tested applicationsIP, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Lysozyme isolated from urine of patients with monocytic leukemia.

  • Positive control
    • In Western Blot, this antibody gave a positive signal in HL60 Whole Cell Lysate and in Human Spleen Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab2408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • Involvement in diseaseDefects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 22 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 4 beta n acetylmuramidase c antibody
    • 1 antibody
    • 4-beta-N-acetylmuramidase C antibody
    • EC 3.2.1.17 antibody
    • LYSC_HUMAN antibody
    • Lysosyme antibody
    • Lysozyme (renal amyloidosis) antibody
    • Lysozyme C antibody
    • Lysozyme C precursor antibody
    • Lyz antibody
    • LZM antibody
    • Renal amyloidosis antibody
    see all

Anti-Lysozyme antibody images

  • Lysozyme was immunoprecipitated using 0.5mg HL60 whole cell extract, 5µg of Rabbit polyclonal to Lysozyme and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, HL60 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2408.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 16kDa; Lysozyme

  • Ab2408 staining lysozyme in human colon. Staining is localised to cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • All lanes : Anti-Lysozyme antibody (ab2408) at 1 µg/ml

    Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Lysozyme with ab2408.

References for Anti-Lysozyme antibody (ab2408)

ab2408 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (human gut mucosa)
Loading amount 30 µg
Specification human gut mucosa
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Apr 03 2013

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please le...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"