Overview

  • Product name
  • Description
    Rabbit polyclonal to Lysozyme
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lysozyme from hen egg white.
  • Tested applications
    Suitable for: WB, IP, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Lysozyme from Hen Egg White

  • Positive control
    • Assayed against 1.0 ug of Lysozyme from hen egg white in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.
IP 1/100.
ELISA 1/25000.
ICC 1/500 - 1/2000.

Target

  • Function
    Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • Involvement in disease
    Amyloidosis 8
  • Sequence similarities
    Belongs to the glycosyl hydrolase 22 family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 4 beta N acetylmuramidase C antibody
    • 1 antibody
    • 4-beta-N-acetylmuramidase C antibody
    • EC 3.2.1.17 antibody
    • LYSC_HUMAN antibody
    • Lysosyme antibody
    • Lysozyme (renal amyloidosis) antibody
    • Lysozyme C antibody
    • Lysozyme C precursor antibody
    • LYZ antibody
    • LZM antibody
    • Renal amyloidosis antibody
    see all

Images

  • Anti-Lysozyme antibody (ab391) at 1/1000 dilution + Lysozyme (Hen Egg White) at 0.05 µg

    Secondary
    Rabbit HRP conjugate at 1/40000 dilution

References

This product has been referenced in:
  • Doulias PT  et al. Mass spectrometry-based identification of S-nitrosocysteine in vivo using organic mercury assisted enrichment. Methods : (2012). WB . Read more (PubMed: 23116708) »
  • Sagiv Y  et al. A distal effect of microsomal triglyceride transfer protein deficiency on the lysosomal recycling of CD1d. J Exp Med 204:921-8 (2007). WB . Read more (PubMed: 17403933) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Chicken Tissue sections (embryo)
Specification
embryo
Permeabilization
No
Fixative
Paraformaldehyde
Username

Anna Keyle

Verified customer

Submitted Jun 12 2014

Thank you for your enquiry. We currently do not have a lysozyme antibody conjugated to AP and we are unable to custom conjugate antibodies.

This antibody has not yet been tested for use with IHC. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher. If you have more questions, please c...

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Yes, this antibody has been tested by immunoprecipitation and Western blot. The suggested working dilution is 1/4000 to 1/10000, although optimisation in your own lab is recommended.

Polyclonal antibodies are normally produced in SPF (specific pathogen free) rabbits and these rabbits are free of infection with E. coli. Even with these precautions, quite a lot of polyclonal antibodies give cross-reaction with E. coli proteins. Try ...

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This antibody is known to work in immunoblotting, however it's reactivity against dog lysozyme has not been determined. As the antibody was raised against lysozyme from chicken egg white, it is uncertain whether it will react with canine.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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