Overview

  • Product nameAnti-Lysozyme antibody
    See all Lysozyme primary antibodies
  • Description
    Rabbit polyclonal to Lysozyme
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human Lysozyme from the urine of patients with monocytic leukemia.

  • Positive control
    • Human tonsil tissue. IF/ICC: A549 cell line.
  • General notes


    ab76784 labels myeloid cells, histocytes, granulocytes, macrophages and monocytes. It is helpful in detection of myeloid or monocytic nature of acute leukemia.

Properties

Applications

Our Abpromise guarantee covers the use of ab76784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
ICC 1/50 - 1/200.

Target

  • FunctionLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • Involvement in diseaseAmyloidosis 8
  • Sequence similaritiesBelongs to the glycosyl hydrolase 22 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 4 beta N acetylmuramidase C antibody
    • 1 antibody
    • 4-beta-N-acetylmuramidase C antibody
    • EC 3.2.1.17 antibody
    • LYSC_HUMAN antibody
    • Lysosyme antibody
    • Lysozyme (renal amyloidosis) antibody
    • Lysozyme C antibody
    • Lysozyme C precursor antibody
    • LYZ antibody
    • LZM antibody
    • Renal amyloidosis antibody
    see all

Anti-Lysozyme antibody images

  • ICC/IF image of ab76784 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76784, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Lysozyme antibody (ab76784)

ab76784 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"