Anti-Lysozyme antibody [BGN/06/961] (ab36362)

Overview

  • Product nameAnti-Lysozyme antibody [BGN/06/961]
    See all Lysozyme primary antibodies
  • Description
    Mouse monoclonal [BGN/06/961] to Lysozyme
  • SpecificityWe have received mixed feedback on the reactivity of this antibody with the chicken protein and are therefore not able to conclude whether this antibody works or not on chicken.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native Lysozyme/ Muramidase protein (purified) (Human urine)

Properties

Applications

Our Abpromise guarantee covers the use of ab36362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 17 kDa.
ELISA 1/5000. (see Abreview)

Target

  • FunctionLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • Involvement in diseaseAmyloidosis 8
  • Sequence similaritiesBelongs to the glycosyl hydrolase 22 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 4 beta N acetylmuramidase C antibody
    • 1 antibody
    • 4-beta-N-acetylmuramidase C antibody
    • EC 3.2.1.17 antibody
    • LYSC_HUMAN antibody
    • Lysosyme antibody
    • Lysozyme (renal amyloidosis) antibody
    • Lysozyme C antibody
    • Lysozyme C precursor antibody
    • LYZ antibody
    • LZM antibody
    • Renal amyloidosis antibody
    see all

Anti-Lysozyme antibody [BGN/06/961] images

  • ab36362 staining Lysozyme (green) in Human lacrimal gland cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and permeabilized with 50% methanol for 20 minutes. Samples were incubated with primary antibody (1/100 in diluent) for 2 hours at room temperature. An FITC-conjugated anti-mouse IgG (1/200) was used as the secondary antibody. Nuclei were stained with propidium iodide (red).
  • ab36362 staining Lysozyme in Human lacrimal gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with formalin and blocked with 2.5% BSA; antigen retrieval was by Tris-EDTA buffer. Samples were incubated with primary antibody (1/100 in diluent) for 2 hours at room temperature. A polymer HRP secondary was used and stained with DAB. Counterstaining was by hematoxylin.

References for Anti-Lysozyme antibody [BGN/06/961] (ab36362)

This product has been referenced in:
  • Lo Sasso G  et al. Loss of Sirt1 function improves intestinal anti-bacterial defense and protects from colitis-induced colorectal cancer. PLoS One 9:e102495 (2014). WB . Read more (PubMed: 25013930) »
  • Zhou WJ  et al. Induction of intestinal stem cells by R-spondin 1 and Slit2 augments chemoradioprotection. Nature 501:107-11 (2013). Read more (PubMed: 23903657) »

See all 8 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Mouse Tissue (duodenum)
Specification duodenum
Username

Abcam user community

Verified customer

Submitted Apr 26 2016

Thank you for contacting us.

Your credit note ID for the refund is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a ref...

Read More

Thank you very much for your call today. My colleague has let me know that she spoke to you about this blocking peptide, and I'm very sorry for the delay.

We are still waiting to receive the vial of the peptide from the lab, and it will be se...

Read More

Thank you very much fory our reply.

I'm sending theblocking peptide free of charge on the order ***, which should arrive early next week. Please keep me updated about the results with this peptide and with the lysozyme antibody in non-reduced...

Read More

Thank you again for your call earlier this week and for letting us know about the trouble with these antibodies.
I have heard from the lab about the protocol used with ab36362, and the sample was prepared in non-reducing buffer, however we have no...

Read More

Thank you very much for your call yesterday and for letting us know about the trouble with these antibodies.

I am currently working to add the blocking peptide of ab66610 to our catalog, and I'll be happy to send a free of charge vial to use ...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I have issued a free of charge replacement with the order number **** for ab108508

To ...

Read More

rr/>
I am sorry to hear you are experiencing difficulties with the anti-Lysozyme antibody [BGN/06/961] (ab36362). As you have been using the antibody successfully in Western blotting it seems that the antibody is functioning properly, but it may b...

Read More

Merci pour votre patience. Malheureusement je n'ai pas encore pu conclure pour savoir quel anticorps sera le meilleur pour réagir contre la protéine de poulet et humaine en même temps. Deux de nos anticorps sont faites contre un peptide (ab1085...

Read More

Merci de nous avoir contactés. Nous sommes désolés d'apprendre que le ab36362 ne fonctionne pas comme attendu sur la protéine de poulet. Nous sommes très désolée de cette erreur et allons vous remplacer le produit. Comme discuté au téléphone, n...

Read More

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"