Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Overview

  • Product nameAnti-Lysozyme antibody [EPR2994(2)]
    See all Lysozyme primary antibodies
  • Description
    Rabbit monoclonal [EPR2994(2)] to Lysozyme
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    corresponding to Human Lysozyme.

  • Positive control
    • HL60, THP-1 cells; RAW 264.7 and HL-60 whole cell lysate; Human spleen tissue lysates; Human tonsil, spleen, lung and kidney tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-Lysozyme antibody (HRP) [EPR2994(2)] (ab197705)

    Anti-Lysozyme antibody (Biotin) [EPR2994(2)] (ab197704)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab108508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.

 For unpurified use at 1/500 - 1/1000.

WB 1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
    • Involvement in diseaseAmyloidosis 8
    • Sequence similaritiesBelongs to the glycosyl hydrolase 22 family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 4 beta N acetylmuramidase C antibody
      • 1 antibody
      • 4-beta-N-acetylmuramidase C antibody
      • EC 3.2.1.17 antibody
      • LYSC_HUMAN antibody
      • Lysosyme antibody
      • Lysozyme (renal amyloidosis) antibody
      • Lysozyme C antibody
      • Lysozyme C precursor antibody
      • LYZ antibody
      • LZM antibody
      • Renal amyloidosis antibody
      see all

    Anti-Lysozyme antibody [EPR2994(2)] images

    • Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 16 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM/TBST
    • Immunohistochemical analysis of paraffin-embedded human lung tissue sections labeling lysozyme with purified ab108508 at a dilution of 1/1500 (0.6 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody.Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia) cells labeling lysozyme with purified ab108508 at 1/250. Cells were fixed with 100% methanol. ab150077, Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 g/mL) - Microtubule Marker (Alexa Fluor®594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue sections labeling lysozyme with purified ab108508 at a dilution of 1/1500 (0.6 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • All lanes : Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution (unpurified)

      Lane 1 : HL60 cell lysate
      Lane 2 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 17 kDa
    • Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

    • Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/50000 dilution (purified) + HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 16 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM/TBST
    • Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

    • Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.

    • Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1 µg/ml (unpurified) + Natural human Lysozyme protein (ab91125) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 17 kDa


      Exposure time : 4 minutes
    • Unpurified ab108508 showing negative staining in Normal brain tissue.

    • Unpurified ab108508 showing negative staining in Normal breast tissue.

    • Unpurified ab108508 showing negative staining in Normal heart tissue.

    References for Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

    This product has been referenced in:
    • Wan X  et al. Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice. Nutrients 7:6294-312 (2015). IHC-P ; Mouse . Read more (PubMed: 26247961) »
    • Riehl TE  et al. CD44 and TLR4 mediate hyaluronic acid regulation of Lgr5+ stem cell proliferation, crypt fission, and intestinal growth in postnatal and adult mice. Am J Physiol Gastrointest Liver Physiol 309:G874-87 (2015). Read more (PubMed: 26505972) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (small intestine)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization No
    Specification small intestine
    Blocking step AB block Dako X0590 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative 10% NBF
    Username

    Herr Dr. Thorsten Cramer

    Verified customer

    Submitted Aug 18 2016

    This antibody would not distinguish between the mouse M or P lysozyme isoforms as the immunogen sequence in conserved in both.

    Thank you for providing that information.

    I have had a look at the two rabbit monoclonal against Lysozyme (ab91653 and ab108508). Both of these antibodies should be able to detect all three forms of the protein (the wild type as well as your ...

    Read More


    Thank you for contacting us yesterday.

    I have received further information from the lab regarding the IHC protocol for ab108508. I can
    confirm that 0.01M Sodium Citrate Buffer, pH 6.0 was used for antigen retrieval. Here is a copy ...

    Read More

    Thank you for contacting us. Based on sequence analysis, immunogen of ab91653 does not share any significant sequence similarity with chicken. For the immunogen of ab108508, it shares 67% identity with chicken Lysozyme C, so it should also be human...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"