Anti-Lysozyme antibody [EPR2994(2)] (ab108508)


  • Product nameAnti-Lysozyme antibody [EPR2994(2)]
    See all Lysozyme primary antibodies
  • Description
    Rabbit monoclonal [EPR2994(2)] to Lysozyme
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Lysozyme.

  • Positive control
    • HL60 and Human spleen lysates; Human tonsil, spleen and kidney tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


    Alternative versions available:

    Anti-Lysozyme antibody (HRP) [EPR2994(2)] (ab197705)

    Anti-Lysozyme antibody (Biotin) [EPR2994(2)] (ab197704)



Our Abpromise guarantee covers the use of ab108508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IP 1/10 - 1/100.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/500 - 1/1000.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
    • Involvement in diseaseAmyloidosis 8
    • Sequence similaritiesBelongs to the glycosyl hydrolase 22 family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 4 beta N acetylmuramidase C antibody
      • 1 antibody
      • 4-beta-N-acetylmuramidase C antibody
      • EC antibody
      • LYSC_HUMAN antibody
      • Lysosyme antibody
      • Lysozyme (renal amyloidosis) antibody
      • Lysozyme C antibody
      • Lysozyme C precursor antibody
      • LYZ antibody
      • LZM antibody
      • Renal amyloidosis antibody
      see all

    Anti-Lysozyme antibody [EPR2994(2)] images

    • All lanes : Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 17 kDa
    • ab108508, at 1/1000 dilution, staining Lysozyme in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
    • ab108508, at 1/1000 dilution, staining Lysozyme in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.
    • ab108508, at 1/1000 dilution, staining Lysozyme in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.
    • Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1 µg/ml + Natural human Lysozyme protein (ab91125) at 0.1 µg

      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 4 minutes
    • ab108508 showing negative staining in Normal brain tissue.

    • ab108508 showing negative staining in Normal breast tissue.

    • ab108508 showing negative staining in Normal heart tissue.

    • ab108508 showing positive staining in Normal lung tissue.

    References for Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

    This product has been referenced in:
    • Wan X  et al. Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice. Nutrients 7:6294-312 (2015). IHC-P ; Mouse . Read more (PubMed: 26247961) »
    • Riehl TE  et al. CD44 and TLR4 mediate hyaluronic acid regulation of Lgr5+ stem cell proliferation, crypt fission, and intestinal growth in postnatal and adult mice. Am J Physiol Gastrointest Liver Physiol 309:G874-87 (2015). Read more (PubMed: 26505972) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (small intestine)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization No
    Specification small intestine
    Blocking step AB block Dako X0590 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative 10% NBF

    Herr Dr. Thorsten Cramer

    Verified customer

    Submitted Aug 18 2016

    This antibody would not distinguish between the mouse M or P lysozyme isoforms as the immunogen sequence in conserved in both.

    Thank you for providing that information.

    I have had a look at the two rabbit monoclonal against Lysozyme (ab91653 and ab108508). Both of these antibodies should be able to detect all three forms of the protein (the wild type as well as your ...

    Read More

    Thank you for contacting us yesterday.

    I have received further information from the lab regarding the IHC protocol for ab108508. I can
    confirm that 0.01M Sodium Citrate Buffer, pH 6.0 was used for antigen retrieval. Here is a copy ...

    Read More

    Thank you for contacting us. Based on sequence analysis, immunogen of ab91653 does not share any significant sequence similarity with chicken. For the immunogen of ab108508, it shares 67% identity with chicken Lysozyme C, so it should also be human...

    Read More