For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
ab112139 offers a sensitive fluorescent assay for detecting the activity of lysyl oxidase. It utilizes a proprietary LOX substrate that releases hydrogen peroxide detected using our proprietary red fluorescence substrate in HRP-coupled reactions. This method allows the detection of sub ng/mL lysyl oxidase and is much more sensitive than the currently available assays. It eliminates the interference that occurs in some biological samples and can be readily used to detect lysyl oxidase activity in cell extracts or solutions. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm.
Visit our FAQs page for tips and troubleshooting.
ab112139 should be stored desiccated.
Please NOTE that a protein standard is not provided with this kit.
|Assay Buffer||1 x 50ml|
|DMSO||1 x 200µl|
|Horseradish Peroxidase||1 vial|
|HRP Substrate||1 vial|
Our Abpromise guarantee covers the use of ab112139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Lysyl Oxidase Activity in cell lysates, O.D. values measured colorimetrically.
Lysyl Oxidase Activity in cell supernatants, O.D. values measured colorimetrically.
Lysyl Oxidase Activity in mouse tissue lysates, RFU values measured fluorometrically.
Lysyl Oxidase Activity in mouse tissue lysates, O.D. values measured colorimetrically.
Lysyl Oxidase RFU values vs quantity (µg) measured fluorometrically.
Lysyl Oxidase O.D. values vs quantity (µg) measured colorimetrically.
ab112139 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"