Lysyl Oxidase Activity Assay Kit (Fluorometric) (ab112139)
- Product nameLysyl Oxidase Activity Assay Kit (Fluorometric)
- Sample typeCell culture extracts, Adherent cells, Suspension cells
- Assay typeDirect
- Sensitivity40 ng/well
- Species reactivityReacts with: Human, Pig
Predicted to work with: all Mammals
- Product overview
ab112139 offers a sensitive fluorescent assay for detecting the activity of lysyl oxidase. It utilizes a proprietary LOX substrate that releases hydrogen peroxide detected using our proprietary red fluorescence substrate in HRP-coupled reactions. This method allows the detection of sub ng/mL lysyl oxidase and is much more sensitive than the currently available assays. It eliminates the interference that occurs in some biological samples and can be readily used to detect lysyl oxidase activity in cell extracts or solutions. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm.
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ab112139 should be stored desiccated.
Please NOTE that a protein standard is not provided with this kit.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 500 tests Assay Buffer 1 x 50ml DMSO 1 x 200µl Horseradish Peroxidase 1 vial HRP Substrate 1 vial
Our Abpromise guarantee covers the use of ab112139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Lysyl Oxidase Activity Assay Kit (Fluorometric) images
ab112139 used to measure Lysyl oxidase dose response on a solid black 96-well with ab112139. As low as 40 ng of lysyl oxidase can be detected with 30 minutes incubation (n=3). The insert shows the low levels of lysyl oxidase detection.
References for Lysyl Oxidase Activity Assay Kit (Fluorometric) (ab112139)
ab112139 has not yet been referenced specifically in any publications.