Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab31532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21460219
IHC-P Use at an assay dependent dilution.
IP 1/3000 - 1/8000.
WB 1/8000. Predicted molecular weight: 74 kDa.

Target

  • FunctionCatalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
  • Involvement in diseaseDefects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family.
  • DomainThe N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
  • Cellular localizationMitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha.
  • Target information above from: UniProt accession Q15046 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CMTRIB antibody
    • DFNB89 antibody
    • EC 6.1.1.6 antibody
    • KARS 1 antibody
    • KARS 2 antibody
    • KARS antibody
    • KARS1 antibody
    • KARS2 antibody
    • KIAA0070 antibody
    • KRS antibody
    • Lysine tRNA ligase antibody
    • Lysine--tRNA ligase antibody
    • LysRS antibody
    • Lysyl-tRNA synthetase antibody
    • SYK_HUMAN antibody
    see all

Anti-Lysyl tRNA synthetase antibody images

  • ab31532 staining Lysyl tRNA synthetase in normal human breast tissue by Immunohistochemistry (paraffin embedded sections).
  • All lanes : Anti-Lysyl tRNA synthetase antibody (ab31532)

    Lane 1 : HEK293 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HCC1208 cell lysate


    Predicted band size : 74 kDa
    Observed band size : 74 kDa

References for Anti-Lysyl tRNA synthetase antibody (ab31532)

This product has been referenced in:
  • Gulati P  et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013). WB, IP ; Mouse . Read more (PubMed: 23359686) »
  • van Meel E  et al. Rare recessive loss-of-function methionyl-tRNA synthetase mutations presenting as a multi-organ phenotype. BMC Med Genet 14:106 (2013). Read more (PubMed: 24103465) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 2 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

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Verified customer

Submitted Jul 08 2011

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 10 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"