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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
Overview
Product name
Anti-Lysyl tRNA synthetase antibody
See all Lysyl tRNA synthetase products (5) ...
Description
Rabbit polyclonal to Lysyl tRNA synthetase
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
A region between residue 550 and the C-terminus (residue 597) of human Lysyl tRNA synthetase (NP_005539.1).
Positive control
Whole cell lysate from HeLa and 293T cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 1 - 4 µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Domain
The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
Cellular localization
Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha.
Target information above from: UniProt accessionQ15046
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 6.1.1.6 antibody
- KARS 2 antibody
- KARS antibody
see all
Database links
- Entrez Gene: 3735 Human
- Omim: 601421 Human
- SwissProt: Q15046 Human
- Unigene: 3100 Human
Anti-Lysyl tRNA synthetase antibody images:
Western blot - Lysyl tRNA synthetase antibody (ab70524)
All lanes : Anti-Lysyl tRNA synthetase antibody (ab70524) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
developed using the ECL technique
Predicted band size : 66 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Immunoprecipitation - Lysyl tRNA synthetase antibody (ab70524)
Detection of Human Lysyl tRNA synthetase by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70524 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
References for Anti-Lysyl tRNA synthetase antibody (ab70524)
ab70524 has not yet been referenced specifically in any publications.
Publishing research using ab70524? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"