Overview

  • Product nameAnti-LYVE1 antibody [4G1]
    See all LYVE1 primary antibodies
  • Description
    Mouse monoclonal [4G1] to LYVE1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human LYVE-1 (25-235 aa) purified from E. coli.

  • Positive control
    • A549 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number4G1
  • MyelomaSp2/0
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab33477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).

Target

  • FunctionLigand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • Tissue specificityMainly expressed in endothelial cells lining lymphatic vessels.
  • Sequence similaritiesContains 1 Link domain.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationMembrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell surface retention sequence-binding protein 1 antibody
    • CRSBP 1 antibody
    • CRSBP-1 antibody
    • CRSBP1 antibody
    • extracellular link domain containing 1 antibody
    • extracellular link domain-containing 1 antibody
    • Extracellular link domain-containing protein 1 antibody
    • HAR antibody
    • Hyaluronic acid receptor antibody
    • Lymphatic endothelium specific hyaluronan receptor antibody
    • lymphatic vessel endothelial hyaluronan receptor 1 antibody
    • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
    • LYVE 1 antibody
    • LYVE-1 antibody
    • LYVE1 antibody
    • LYVE1_HUMAN antibody
    • XLKD1 antibody
    see all

Anti-LYVE1 antibody [4G1] images

  • Anti-LYVE1 antibody [4G1] (ab33477) at 1/500 dilution + A549 (lung carcinoma) cell lysate

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • ICC/IF analysis of LYVE-1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human LYVE-1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

References for Anti-LYVE1 antibody [4G1] (ab33477)

ab33477 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"