Overview

  • Product nameAnti-LYVE1 antibody
    See all LYVE1 primary antibodies
  • Description
    Rabbit polyclonal to LYVE1
  • Tested applicationsSuitable for: ELISA, WB, IHC-Fr, ICC/IF, Flow Cyt, IHC - Wholemount, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
  • Immunogen

    Recombinant fragment corresponding to LYVE1.

Properties

Applications

Our Abpromise guarantee covers the use of ab14917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
IHC-Fr Use a concentration of 1 - 5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22590615
Flow Cyt Use at an assay dependent concentration. PubMed: 22590615

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC - Wholemount Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionLigand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • Tissue specificityMainly expressed in endothelial cells lining lymphatic vessels.
  • Sequence similaritiesContains 1 Link domain.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationMembrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell surface retention sequence-binding protein 1 antibody
    • CRSBP 1 antibody
    • CRSBP-1 antibody
    • CRSBP1 antibody
    • extracellular link domain containing 1 antibody
    • extracellular link domain-containing 1 antibody
    • Extracellular link domain-containing protein 1 antibody
    • HAR antibody
    • Hyaluronic acid receptor antibody
    • Lymphatic endothelium specific hyaluronan receptor antibody
    • lymphatic vessel endothelial hyaluronan receptor 1 antibody
    • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
    • LYVE 1 antibody
    • LYVE-1 antibody
    • LYVE1 antibody
    • LYVE1_HUMAN antibody
    • XLKD1 antibody
    see all

Anti-LYVE1 antibody images

  • ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.

    This image is courtesy of an Abreview submitted by Elizabeth Chlipala on 18 November 2005.

    See Abreview

  •  ab14917 at 1/100 staining mouse tissue sections (E17.5 lymphatic vessels by inferior vena cava) by IHC-Fr. The tissue was acetone fixed and incubated with the antibody for 2 hours in 1% BSA in PBS. An Alexa Fluor ® 488 conjugated donkey anti-rabbit IgG antibody was used as the secondary.

    See Abreview

  • ab14917 staining LYVE1 in Rabbit spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in Fa.Zytomed) for 24 hours at 4°C. An undiluted Biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab14917 staining LYVE1 in Mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-Buffer pH9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.

    See Abreview

References for Anti-LYVE1 antibody (ab14917)

This product has been referenced in:
  • Nemska S  et al. Revascularization of the graft in obliterative bronchiolitis after heterotopic tracheal transplantation. Physiol Rep 4:N/A (2016). IHC-P ; Mouse . Read more (PubMed: 26908711) »
  • Kwon JW  et al. Comparison of postoperative corneal changes between dry eye and non-dry eye in a murine cataract surgery model. Int J Ophthalmol 9:218-24 (2016). IHC ; Mouse . Read more (PubMed: 26949638) »

See all 101 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application IHC - Wholemount
Sample Mouse Embryo (dermal lymphatic vessels)
Specification dermal lymphatic vessels
Username

Abcam user community

Verified customer

Submitted Dec 14 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Lungs)
Permeabilization Yes - 0.5% TX100
Specification Lungs
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Oct 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse embryo, E14.5)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Specification Mouse embryo, E14.5
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Molly Plehaty

Verified customer

Submitted Sep 15 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 27 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Syrian Hamster Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 22 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (jejunum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification jejunum
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 22 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Uterus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification Uterus
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 21 2015

Application Western blot
Sample Mouse Tissue lysate - whole (Heart and brain)
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount 10 µg
Treatment Tissue was mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification Heart and brain
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

Submitted Apr 27 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample Pig Tissue sections (Lung Biopsy)
Specification Lung Biopsy
Permeabilization No
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

Submitted Mar 11 2015

1-10 of 39 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"