Overview

  • Product name
  • Description
    Rabbit polyclonal to LYVE1
  • Tested applications
    Suitable for: ELISA, WB, IHC-Fr, ICC/IF, Flow Cyt, IHC - Wholemount, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
  • Immunogen

    Recombinant fragment corresponding to LYVE1.

Properties

Applications

Our Abpromise guarantee covers the use of ab14917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
IHC-Fr Use a concentration of 1 - 5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22590615
Flow Cyt Use at an assay dependent concentration. PubMed: 22590615

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC - Wholemount Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • Tissue specificity
    Mainly expressed in endothelial cells lining lymphatic vessels.
  • Sequence similarities
    Contains 1 Link domain.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localization
    Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell surface retention sequence-binding protein 1 antibody
    • CRSBP 1 antibody
    • CRSBP-1 antibody
    • CRSBP1 antibody
    • extracellular link domain containing 1 antibody
    • extracellular link domain-containing 1 antibody
    • Extracellular link domain-containing protein 1 antibody
    • HAR antibody
    • Hyaluronic acid receptor antibody
    • Lymphatic endothelium specific hyaluronan receptor antibody
    • lymphatic vessel endothelial hyaluronan receptor 1 antibody
    • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
    • LYVE 1 antibody
    • LYVE-1 antibody
    • LYVE1 antibody
    • LYVE1_HUMAN antibody
    • XLKD1 antibody
    see all

Anti-LYVE1 antibody images

  • ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.

    This image is courtesy of an Abreview submitted by Elizabeth Chlipala on 18 November 2005.

    See Abreview

  •  ab14917 at 1/100 staining mouse tissue sections (E17.5 lymphatic vessels by inferior vena cava) by IHC-Fr. The tissue was acetone fixed and incubated with the antibody for 2 hours in 1% BSA in PBS. An Alexa Fluor ® 488 conjugated donkey anti-rabbit IgG antibody was used as the secondary.

    See Abreview

  • ab14917 staining LYVE1 in Rabbit spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in Fa.Zytomed) for 24 hours at 4°C. An undiluted Biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab14917 staining LYVE1 in Mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-Buffer pH9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.

    See Abreview

References for Anti-LYVE1 antibody (ab14917)

This product has been referenced in:
  • Gurung HR  et al. Cornea lymphatics drive the CD8(+) T-cell response to herpes simplex virus-1. Immunol Cell Biol 95:87-98 (2017). ICC/IF ; Mouse . Read more (PubMed: 27577867) »
  • Strömvall K  et al. Aggressive rat prostate tumors reprogram the benign parts of the prostate and regional lymph nodes prior to metastasis. PLoS One 12:e0176679 (2017). IHC-Fr ; Rat . Read more (PubMed: 28472073) »

See all 104 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Infmammed lung tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
No
Specification
Infmammed lung tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative
Paraformaldehyde
Username

Dr. Jim Hsiao

Verified customer

Submitted Jun 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Mouse Embryo (dermal lymphatic vessels)
Specification
dermal lymphatic vessels
Username

Abcam user community

Verified customer

Submitted Dec 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lungs)
Permeabilization
Yes - 0.5% TX100
Specification
Lungs
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Oct 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse embryo, E14.5)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
Mouse embryo, E14.5
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Molly Plehaty

Verified customer

Submitted Sep 15 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 27 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Syrian Hamster Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 22 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization
No
Specification
jejunum
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 22 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization
No
Specification
Uterus
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 21 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart and brain)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
10 µg
Treatment
Tissue was mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
Heart and brain
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

Submitted Apr 27 2015

1-10 of 40 Abreviews or Q&A

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