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Product name
Anti-LYVE1 antibody - Lymphatic Vessel Marker
See all LYVE1 products (8) ...
Description
Rabbit polyclonal to LYVE1 - Lymphatic Vessel Marker
Tested applications
ELISA, WB, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Recombinant mouse soluble LYVE1, consisting of amino acids 24 to 228 fused to a C-terminal His-tag (6xHis), produced in insect cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Protein-A Chromatography (+his tag depleted).
Clonality
Polyclonal
Isotype
IgG
Research areas
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Immunohistochemistry (Paraffin-embedded sections) - LYVE1 antibody - Lymphatic Vessel Marker (ab14917)
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Immunohistochemistry (Paraffin-embedded sections) - LYVE1 antibody - Lymphatic Vessel Marker (ab14917)
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Immunohistochemistry (Frozen sections) - LYVE1 antibody - Lymphatic Vessel Marker (ab14917)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab14917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Application notes
ELISA: Use at a concentration of 1 - 15 µg/ml.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at a concentration of 1 - 5 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
Tissue specificity
Mainly expressed in endothelial cells lining lymphatic vessels.
Sequence similarities
Contains 1 Link domain.
Post-translational
modifications
O-glycosylated.
Cellular localization
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
Target information above from: UniProt accessionQ9Y5Y7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell surface retention sequence-binding protein 1 antibody
- CRSBP 1 antibody
- CRSBP-1 antibody
see all
Database links
- Entrez Gene: 114332 Mouse
- SwissProt: Q99NE4 Mouse
- SwissProt: Q8BHC0 Mouse
- Unigene: 396078 Mouse
Anti-LYVE1 antibody - Lymphatic Vessel Marker images:
Immunohistochemistry (Paraffin-embedded sections) - LYVE1 antibody - Lymphatic Vessel Marker (ab14917)
ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.
This image is courtesy of an Abreview submitted by Elizabeth Chlipala on 18 November 2005.
Immunohistochemistry (Paraffin-embedded sections) - LYVE1 antibody - Lymphatic Vessel Marker (ab14917)
ab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Frozen sections) - LYVE1 antibody - Lymphatic Vessel Marker (ab14917)
ab14917 at 1/100 staining mouse tissue sections (E17.5 lymphatic vessels by inferior vena cava) by IHC-Fr. The tissue was acetone fixed and incubated with the antibody for 2 hours in 1% BSA in PBS. An Alexa Fluor ® 488 conjugated donkey anti-rabbit IgG antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Miss Lise Sorensen
References for Anti-LYVE1 antibody - Lymphatic Vessel Marker (ab14917)
This product has been referenced in:
- Boimel PJ et al. fContribution of CXCL12 secretion to invasion of breast cancer cells. Breast Cancer Res 14:R23 (2012). IHC-P.Read more (PubMed: 22314082) »
- Martínez-Corral I et al. In vivo imaging of lymphatic vessels in development, wound healing, inflammation, and tumor metastasis. Proc Natl Acad Sci U S A 109:6223-8 (2012). IHC (PFA fixed); Mouse.Read more (PubMed: 22474390) »
See all 31 publications for this product
Publishing research using ab14917? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"