Overview

  • Product nameAnti-M Cadherin antibody
    See all M Cadherin primary antibodies
  • Description
    Rabbit polyclonal to M Cadherin
  • Specificityab65157 detects endogenous levels of total M Cadherin protein.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human M Cadherin

  • Positive control
    • HepG2 and 293 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab65157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20723755
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).

Target

  • FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation.
  • Tissue specificityExpressed in the brain and cerebellum.
  • Involvement in diseaseA chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
    Mental retardation, autosomal dominant 3
  • Sequence similaritiesContains 5 cadherin domains.
  • DomainThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAD15_HUMAN antibody
    • Cadherin 14 antibody
    • Cadherin 15 antibody
    • Cadherin 15, type 1, M cadherin (myotubule) antibody
    • Cadherin 3 antibody
    • Cadherin-14 antibody
    • Cadherin-15 antibody
    • Cadherin14 antibody
    • Cadherin15 antibody
    • Cadherin3 antibody
    • CCAD antibody
    • CDH 14 antibody
    • CDH 15 antibody
    • CDH 3 antibody
    • CDH14 antibody
    • Cdh15 antibody
    • CDH3 antibody
    • CDHM antibody
    • M-cadherin antibody
    • MCAD antibody
    • MRD3 antibody
    • Muscle cadherin antibody
    • Myotubule cadherin antibody
    see all

Anti-M Cadherin antibody images

  • All lanes : Anti-M Cadherin antibody (ab65157) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : 293 cell extract
    Lane 3 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 90 kDa
    Observed band size : 90 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References for Anti-M Cadherin antibody (ab65157)

This product has been referenced in:
  • Rezk BM  et al. Angiotensin II Infusion Induces Marked Diaphragmatic Skeletal Muscle Atrophy. PLoS One 7:e30276 (2012). WB ; Mouse . Read more (PubMed: 22276172) »
  • Zhou X  et al. Reversal of cancer cachexia and muscle wasting by ActRIIB antagonism leads to prolonged survival. Cell 142:531-43 (2010). ICC/IF ; Mouse . Read more (PubMed: 20723755) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"