Overview

  • Product nameAnti-M Cadherin antibody
    See all M Cadherin primary antibodies
  • Description
    Rabbit polyclonal to M Cadherin
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human M Cadherin.

  • Positive control
    • This antibody gave a positive signal in Mouse brain cerebellum tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab87374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation.
  • Tissue specificityExpressed in the brain and cerebellum.
  • Involvement in diseaseA chromosomal aberration involving CDH15 and KIRREL3 is found in a patient with severe mental retardation and dysmorphic facial features. Translocation t(11;16)(q24.2;q24).
    Mental retardation, autosomal dominant 3
  • Sequence similaritiesContains 5 cadherin domains.
  • DomainThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAD15_HUMAN antibody
    • Cadherin 14 antibody
    • Cadherin 15 antibody
    • Cadherin 15, type 1, M cadherin (myotubule) antibody
    • Cadherin 3 antibody
    • Cadherin-14 antibody
    • Cadherin-15 antibody
    • Cadherin14 antibody
    • Cadherin15 antibody
    • Cadherin3 antibody
    • CCAD antibody
    • CDH 14 antibody
    • CDH 15 antibody
    • CDH 3 antibody
    • CDH14 antibody
    • Cdh15 antibody
    • CDH3 antibody
    • CDHM antibody
    • M-cadherin antibody
    • MCAD antibody
    • MRD3 antibody
    • Muscle cadherin antibody
    • Myotubule cadherin antibody
    see all

Anti-M Cadherin antibody images

  • Anti-M Cadherin antibody (ab87374) at 1 µg/ml + Cerebellum Mouse Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 89 kDa
    Observed band size : 89 kDa


    Exposure time : 5 minutes
  • ICC/IF image of ab87374 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab87374 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-M Cadherin antibody (ab87374)

ab87374 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Heart)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 40 µg
Specification Mouse Heart
Blocking step Seablock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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Submitted Jul 25 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse embryo section)
Specification mouse embryo section
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Mar 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"