Overview

  • Product nameAnti-M-CSF antibody
    See all M-CSF primary antibodies
  • Description
    Rabbit polyclonal to M-CSF
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Dog, Pig, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human MCSF.

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Bone Marrow; Spleen; Thymus; Tonsil. It also gave a positive result in FFPE human tonsil tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab99178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).

Target

  • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • Post-translational
    modifications
    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • Cellular localizationCell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Colony stimulating factor 1 (macrophage) antibody
    • Colony stimulating factor 1 antibody
    • Colony stimulating factor macrophage specific antibody
    • CSF 1 antibody
    • CSF-1 antibody
    • CSF1 antibody
    • CSF1_HUMAN antibody
    • Csfm antibody
    • Lanimostim antibody
    • M CSF antibody
    • M-CSF antibody
    • Macrophage Colony Stimulating Factor 1 antibody
    • Macrophage colony stimulating factor antibody
    • MCSF antibody
    • MGC31930 antibody
    • Processed macrophage colony-stimulating factor 1 antibody
    see all

Anti-M-CSF antibody images

  • All lanes : Anti-M-CSF antibody (ab99178) at 1 µg/ml

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)
    Lane 4 : Tonsil (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 170 kDa,52 kDa (possible cleavage fragment),98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    MCSF contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • IHC image of MCSF staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99178, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ab99178 staining MCSF in mouse skin wound tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate-EDTA buffer. Samples were incubated with primary antibody (1/100 in TBS pH 7.6) for 1 hour at 25°C. A Biotin-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/300) was used as the secondary antibody.

    See Abreview

References for Anti-M-CSF antibody (ab99178)

ab99178 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Wound)
Permeabilization No
Specification Wound
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted May 06 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate-EDTA Buffer
Sample Mouse Tissue sections (skin wound healing tissue)
Specification skin wound healing tissue
Permeabilization Yes - 0.1% triton-X100 for 10 min before blocking step
Fixative Paraformaldehyde
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Submitted Jun 16 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Sample Mouse Tissue sections (C57BL6J Testis (adult))
Specification C57BL6J Testis (adult)
Permeabilization No
Fixative Bouin's
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Submitted Dec 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"