- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-M-CSF antibody (Biotin)
See all M-CSF primary antibodies -
DescriptionRabbit polyclonal to M-CSF (Biotin)
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ConjugationBiotin
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Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Highly pure (>98%) recombinant MCSF fragment (Human).
Properties
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FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab84262 is purified by affinity chromatography and then biotinylated and is sterile filtered.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab84262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human MCSF. | |
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to M-CSF (ab9693). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human MCSF. Can be used as detection antibody when paired with ab9693. | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 60 kDa. The detection limit for recombinant human MCSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
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Post-translational
modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
Cellular localizationCell membrane and Secreted > extracellular space.
- Information by UniProt
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Database links
- Entrez Gene: 1435 Human
- Omim: 120420 Human
- SwissProt: P09603 Human
- Unigene: 591402 Human
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Alternative names
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
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Protocols
References for Anti-M-CSF antibody (Biotin) (ab84262)
ab84262 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"