The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000. Predicted molecular weight: 60 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/250 - 1/500.
Application notesIs unsuitable for Flow Cyt.
FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Post-translational modificationsGlycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Cellular localizationCell membrane and Secreted > extracellular space.
References for Anti-M-CSF antibody [EP1179Y] (ab52864)
This product has been referenced in:
Yang L et al. Increased expression of colony stimulating factor-1 is a predictor of poor prognosis in patients with clear-cell renal cell carcinoma. BMC Cancer15:67 (2015).
Read more (PubMed: 25886010) »
Wang YW et al. Clinicopathological significance of microRNA-214 in gastric cancer and its effect on cell biological behaviour. PLoS One9:e91307 (2014).
Read more (PubMed: 24614175) »