Recombinant
RabMAb

Anti-M-CSF antibody [EP1179Y] (ab52864)

Overview

  • Product nameAnti-M-CSF antibody [EP1179Y]
    See all M-CSF primary antibodies
  • Description
    Rabbit monoclonal [EP1179Y] to M-CSF
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Cow, Chimpanzee
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human M-CSF aa 50-150. The exact sequence is proprietary.
    Database link: P09603

  • Positive control
    • WB: Recombinant MCSF IHC-P: Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab52864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Predicted molecular weight: 60 kDa.
IP 1/50.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
    • Post-translational
      modifications
      Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
      Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
    • Cellular localizationCell membrane and Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Colony stimulating factor 1 (macrophage) antibody
      • Colony stimulating factor 1 antibody
      • Colony stimulating factor macrophage specific antibody
      • CSF 1 antibody
      • CSF-1 antibody
      • CSF1 antibody
      • CSF1_HUMAN antibody
      • Csfm antibody
      • Lanimostim antibody
      • M CSF antibody
      • M-CSF antibody
      • Macrophage Colony Stimulating Factor 1 antibody
      • Macrophage colony stimulating factor antibody
      • MCSF antibody
      • MGC31930 antibody
      • Processed macrophage colony-stimulating factor 1 antibody
      see all

    Anti-M-CSF antibody [EP1179Y] images

    • ab52864 at 1/50 dilution stainng MCSF in human tonsil by Immunohistochemistry, Paraffin embedded tissue.
    • Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/50000 dilution + recombinant MCSF at 0.005 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)

    References for Anti-M-CSF antibody [EP1179Y] (ab52864)

    This product has been referenced in:
    • Yang L  et al. Increased expression of colony stimulating factor-1 is a predictor of poor prognosis in patients with clear-cell renal cell carcinoma. BMC Cancer 15:67 (2015). IHC-P ; Human . Read more (PubMed: 25886010) »
    • Wang YW  et al. Clinicopathological significance of microRNA-214 in gastric cancer and its effect on cell biological behaviour. PLoS One 9:e91307 (2014). WB ; Human . Read more (PubMed: 24614175) »

    See all 12 Publications for this product

    Product Wall

    I can confirm to you that it is reported in literature that M-CSF can also be detected in a 45 kDa form (see links below)

    https://www.ncbi.nlm.nih.gov/pubmed/2179407

    https://www.researchgate.net/publication/14333823_Macrophage_colony_...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"