Anti-M13 + Fd Bacteriophage Coat Proteins antibody (ab6188)

Overview

  • Product name
    Anti-M13 + Fd Bacteriophage Coat Proteins antibody
  • Description
    Rabbit polyclonal to M13 + Fd Bacteriophage Coat Proteins
  • Specificity
    Binds specifically to phage coat proteins of M13 or fd phage.
  • Tested applications
    Suitable for: Indirect ELISA, ELISA, ICC/IFmore details
  • Immunogen

    fd bacteriophage

  • General notes
    Concentration: 5.5 mg/ml- 7.5 mg/ml


    Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration. At least 1/1000 for 5 x107 phage/mlat least 1/8000 for 5 x 1010 phage/ml.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 17178915

Target

  • Relevance
    M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends.
  • Alternative names
    • Coat protein A antibody
    • Coat protein B antibody
    • Coat protein C polypeptide I antibody
    • Coat protein C polypeptide II antibody
    • Coat protein D antibody
    • M13 coat protein A antibody
    • M13 coat protein B antibody
    • M13 coat protein C polypeptide I antibody
    • M13 coat protein C polypeptide II antibody
    • M13 coat protein D antibody
    • M13 minor coat protein antibody
    • Minor coat protein antibody
    see all

References

This product has been referenced in:
  • Phipps ML  et al. Beyond Helper Phage: Using "Helper Cells" to Select Peptide Affinity Ligands. PLoS One 11:e0160940 (2016). Read more (PubMed: 27626637) »
  • Vargas-Sanchez K  et al. DNA Subtraction of In Vivo Selected Phage Repertoires for Efficient Peptide Pathology Biomarker Identification in Neuroinflammation Multiple Sclerosis Model. Biomark Insights 11:19-29 (2016). Read more (PubMed: 26917946) »

See all 8 Publications for this product

Customer reviews and Q&As

Application
ELISA
Sample
Bacteria Recombinant protein (filamentous bacteriophage from host E. coli strain)
Specification
filamentous bacteriophage from host E. coli strain
Blocking step
BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type
Indirect
Username

Mr. Chang Su Jeon

Verified customer

Submitted Jul 27 2015

Unfortunately lot 861626 is an individual batch which was not produced from a parent batch, also I'm afraid that we no longer have stock of this batch in Europe or the USA. Sorry that I don't have better news; if you have any further questions please d...

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Thank you very much for your inquiry. The immunogen for this polyclonal antibody was a cell preparation of fd bacteriophage. We therefore do not know what epitope is bound exactly by this antibody. Therefore we also cannot make any predictions about th...

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Thank you for contacting us. We have two anti-M13 antibodies that may be of interest: ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody Binds specifically to phage coat proteins of M13 or fd phage. ...

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Thank you for your reply. We have a number of antibodies to Bacteriophage Proteins such as ab6188: Anti-M13 + Fd Bacteriophage Coat Proteins antibody or ab9225: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]. I hope this he...

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Application
ELISA
Sample
Rabbit Recombinant protein (phage coat proteins of M13)
Specification
phage coat proteins of M13
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 27°C
Type
Sandwich (Capture)
Username

Miss. I-Hsuan Chen

Verified customer

Submitted Jul 09 2010

Thank you for your enquiry. I have been in correspondence with the source of this antibody. Following discussion we concluded that there may be a degree of contamination in your samples. There is clear specific reactivity with your M13- cells. Can ...

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Thank you for your enquiry. We have not tested the stability of the antibody ab6188 when stored in other buffer than its storage buffer so we do not know if it will improve or damage its storage conditions. In my experience it is best to use glyc...

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Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

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I apologise for taking so long I have been awaiting confirmation of the concentration. The concentration of the product is batch dependent and will range from 5.5 mg/ml to 7.5 mg/ml. I hope that this information is useful to you. If I can be of any fur...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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