Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.
Our Abpromise guarantee covers the use of ab6188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration. At least 1/1000 for 5 x107 phage/mlat least 1/8000 for 5 x 1010 phage/ml.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17178915|