Overview

  • Product name
    Anti-M13 antibody [B62-FE2] (HRP)
    See all M13 primary antibodies
  • Description
    Mouse monoclonal [B62-FE2] to M13 (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    ab50370 was raised using fd phages from E. Coli F+ strain (JM109).

  • Epitope
    ab50370 binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT.
  • General notes
    Strongly recommend adding 0.5% BSA to diluents and a suitable preservative (as 0.05-0.09% sodium azide or equivalent) for extra stablity when stored at 2-8 °C


    Filamentous fd bacteriophages are used in immunoassays for the identification of recombinant antigen- or antibody phages. They have also been used to construct phage-display peptide libraries, which have been useful in selecting peptides that interact with specific domains within target molecules.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.40
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Filamentous fd bacteriophages are used in immunoassays for the identification of recombinant antigen- or antibody phages. They have also been used to construct phage-display peptide libraries, which have been useful in selecting peptides that interact with specific domains within target molecules.
  • Clonality
    Monoclonal
  • Clone number
    B62-FE2
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: 1/20.
    ab50370 can be used inPhage Display (immunoassays for the identification of recombinant antigen or antibody-phages) (Detection Limit: 107 phage particles)

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
    • Alternative names
      • Bacteriophage M13 antibody
      • Coliphage M13 antibody
      • Enterobacteria phage M13 antibody
      • M13 virus antibody
      • Phage M13 antibody
      see all

    Images

    • ab50370 detecting M13 phage. Samples were blocked with 1% BSA for 2 hours at 25°C and incubated with the primary antibody (1/1000 in 50mM carbonate/bicarbonate buffer, pH 9) for 12 hours at 4°C.

      See Abreview

    References

    This product has been referenced in:
    • Jung HS  et al. Monoclonal antibodies against autocrine motility factor suppress gastric cancer. Oncol Lett 13:4925-4932 (2017). Read more (PubMed: 28599497) »
    • Shanmugam A  et al. Synthetic Toll like receptor-4 (TLR-4) agonist peptides as a novel class of adjuvants. PLoS One 7:e30839 (2012). ELISA . Read more (PubMed: 22363498) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us.

    The dilution of 1/20 is correct. 250 µl should be used in 5 mL of working solution. When using a volume of 100 µl/well the product allows for testing of 50 wells.

    Please note, the recommended d...

    Read More

    Thank you for contacting us and your interest in our products.

    I am currently contacting the source of the Anti-M13 antibody [B62-FE2] (HRP) (ab50370) to ascertain if the concentration of this antibody has been determined and I will get back ...

    Read More
    Application
    ELISA
    Sample
    Mouse Cell (M13 KE bacteriophage)
    Specification
    M13 KE bacteriophage
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Sandwich (Detection)
    Username

    Abcam user community

    Verified customer

    Submitted Oct 18 2016

    It appears that ab50370 would be best suited to your needs as it is both HRP conjugated and has been tested and shown to work in ELISA.

    Thank you for contacting us and for your interest in our products.

    I am sorry to confirm that as far as we are aware, ab50370 has never been tested in WB . All tested applications covered by our 6 month guaranteeare specified on our datashee...

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    Thank you for your recent enquiries which have been forwarded to me as my colleague Karin is currently away from the office.

    I am pleased to let you know we have received further information regarding the concentration for ab50370. This is c...

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    Thank you for contacting us. We have two anti-M13 antibodies that may be of interest: ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody Binds specifically to phage coat proteins of M13 or fd phage. ...

    Read More

    Thank you for your reply. Our HRP-conjugated M13 antibody ab50370 binds to an epitope on pVIII covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT. I hope this helps, please let me know if you need any additional information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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