Anti-M13 antibody [E1] (ab24229)

Overview

  • Product nameAnti-M13 antibody [E1]
    See all M13 primary antibodies
  • Description
    Mouse monoclonal [E1] to M13
  • SpecificityAntibody specific for gp3 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with M13 Bacteriophage
  • Immunogen

    M13 bacteriophage.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purification notesPurified over protein A/G column.
  • ClonalityMonoclonal
  • Clone numberE1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
    Sorting of M13 infected cells.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
    • Alternative names
      • Bacteriophage M13 antibody
      • Coliphage M13 antibody
      • Enterobacteria phage M13 antibody
      • M13 virus antibody
      • Phage M13 antibody
      see all

    References for Anti-M13 antibody [E1] (ab24229)

    ab24229 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application ELISA
    Sample Bacteria Recombinant protein (filamentous bacteriophage from host E. coli strain)
    Specification filamentous bacteriophage from host E. coli strain
    Blocking step BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type Indirect
    Username

    Mr. Chang Su Jeon

    Verified customer

    Submitted Aug 13 2015

    Application Immunocytochemistry
    Sample Bacteria Cultured Cells (Phage Suspension)
    Permeabilization No
    Specification Phage Suspension
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative none
    Username

    Abcam user community

    Verified customer

    Submitted Oct 06 2015

    Yes, the antibody does detect intact phage, at the gp3 protein.

    Thank you for your enquiry. Regarding ab24229 (M13 antibody [E1]), this antibody is specific for p3 but we do not have more epitope information at this time.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"