Overview

  • Product name
    Anti-M13 antibody [E1] (Biotin)
    See all M13 primary antibodies
  • Description
    Mouse monoclonal [E1] to M13 (Biotin)
  • Conjugation
    Biotin
  • Specificity
    M13 antibody [E1] (ab17269) is specific for gp3 protein.
  • Tested applications
    Suitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity
    M13 antibody [E1] (ab17269) is non-species specific. Not yet tested in other species.
  • Immunogen

    Full length M13 phage coat protein; Exact epitope not determined.

  • General notes


    M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    M13 antibody [E1] (ab17269) is purified using 0.2µm sterile filter.
  • Primary antibody notes
    M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
  • Clonality
    Monoclonal
  • Clone number
    E1
  • Myeloma
    unknown
  • Isotype
    IgG2a
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 90 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
  • Alternative names
    • Bacteriophage M13 antibody
    • Coliphage M13 antibody
    • Enterobacteria phage M13 antibody
    • M13 virus antibody
    • Phage M13 antibody
    see all

References

ab17269 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab17269.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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