Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph1] (ab9223)

Overview

  • Product nameAnti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph1]
    See all M13 Bacteriophage Coat Protein g8p primary antibodies
  • Description
    Mouse monoclonal [RL-ph1] to M13 Bacteriophage Coat Protein g8p
  • SpecificityThis antibody reacts with the M13 filamentous phage coat protein g8p.
  • Tested applicationsSuitable for: Flow Cyt, WB, ICC/IF, ELISA, IHC-Fr, Purificationmore details
  • Immunogen

    Isolated M13 phage coat proteins

  • General notes


    The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThe display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
  • ClonalityMonoclonal
  • Clone numberRL-ph1
  • MyelomaSp2/0-Ag14
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/25 - 1/200. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/100 - 1/1000.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr 1/25 - 1/200.
Purification Use at an assay dependent concentration. Affinity chromatography

Target

  • RelevanceM13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
  • Alternative names
    • Capsid protein G8P antibody
    • Coat protein B antibody
    • G8P antibody
    • Major coat protein antibody
    • VIII antibody
    see all

References for Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph1] (ab9223)

This product has been referenced in:
  • Nemudraya AA  et al. Tumor-Specific Peptide, Selected from a Phage Peptide Library, Enhances Antitumor Activity of Lactaptin. PLoS One 11:e0160980 (2016). Flow Cyt ; Human . Read more (PubMed: 27513518) »
  • Meulemans EV  et al. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas 6:113-8 (1995). Read more (PubMed: 8597626) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. To our knowledge the epitope which is recognized by Rl-ph1 is not known. The antibody response which led to the induction of Rl-ph1 was elicited against a total phage M13 extract and not to a specific epitope of the g8p ...

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Thank you for your enquiry. Is your customer using this antibody in Western blotting or in dot blot? The antibody has only so far been tested for application in Western blotting and Immunohistochemistry (Frozen sections). I do need some additional ...

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In all three cases, all the information we have is on the datasheet. The epitope for each of these antibodies has not been mapped and all are supplied as tissue culture supernatents. We recommend the researcher to optimise conditions for ELISA and WB.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"