Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

Overview

  • Product nameAnti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]
    See all M13 Bacteriophage Coat Protein g8p primary antibodies
  • Description
    Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p
  • SpecificityThis antibody reacts with the M13 filamentous phage coat protein g8p.
  • Tested applicationsSuitable for: ICC, Flow Cyt, ELISA, WBmore details
  • Immunogen

    Isolated M13 phage coat proteins

  • General notes


    The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThe display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
  • ClonalityMonoclonal
  • Clone numberRL-ph2
  • MyelomaSp2/0-Ag14
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt 1/25 - 1/200. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB 1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).

Target

  • RelevanceM13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
  • Alternative names
    • Capsid protein G8P antibody
    • Coat protein B antibody
    • G8P antibody
    • Major coat protein antibody
    • VIII antibody
    see all

Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] images

  • Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution + whole cell lysate prepared from XL10Gold cells at 50 µg

    Secondary
    Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution

    Predicted band size : 5 kDa
    Observed band size : 6 kDa (why is the actual band size different from the predicted?)

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225)

This product has been referenced in:
  • Meulemans EV  et al. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas 6:113-8 (1995). Read more (PubMed: 8597626) »
  • Meulemans EV  et al. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol Biol 244:353-60 (1994). Read more (PubMed: 7527464) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry and your interest. Currently, we only have antibodies against M13 Bacteriophage Coat Protein g8p but not the purified protein. If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for contacting Abcam.

I am sorry to hear of the issues that you have been experimenting with this latest lot of ab9225. I have had the opportunity to speak with my collegues about adding the lateral flow application to our Abreview...

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Thank you for contacting us. Unfortunately we currently do not have any M13 Bacteriophage Coat g8p protein in our catalogue. I'm sorry I could not have been of more help but if you have any further questions please do not hesitate to ask.

Thank you for your reply. We have a number of antibodies to Bacteriophage Proteins such as ab6188: Anti-M13 + Fd Bacteriophage Coat Proteins antibody or ab9225: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]. I hope this he...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Cell lysate - whole cell (XL10Gold)
Loading amount 50 µg
Specification XL10Gold
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Escherichia coli Cell (XL10Gold)
Specification XL10Gold
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type Direct
Username

Abcam user community

Verified customer

Submitted Apr 07 2009

Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"