Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (ab12894)

Overview

  • Product nameAnti-M6PR (cation independent) antibody - Lysosome Membrane Marker
    See all M6PR (cation independent) primary antibodies
  • Description
    Rabbit polyclonal to M6PR (cation independent) - Lysosome Membrane Marker
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, African Green Monkey
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from residues 700 - 800 of Human Mannose 6 Phospate Receptor.

    .

  • Positive control
    • This antibody gave a positive signal in the following Methanol fixed cell lines: MCF-7 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab12894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 4 µg/ml. This antibody worked well in IF in HeLa cells but not in SK-N-SH cells.

Target

  • FunctionTransport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
  • Sequence similaritiesBelongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.
  • DomainContains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
  • Cellular localizationLysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • 300 kDa mannose 6 phosphate receptor antibody
    • 300 kDa mannose 6-phosphate receptor antibody
    • Cation independent mannose 6 phosphate receptor antibody
    • Cation-independent mannose-6-phosphate receptor antibody
    • CD222 antibody
    • CD222 antigen antibody
    • CI Man 6 P receptor antibody
    • CI Man-6-P receptor antibody
    • CI MPR antibody
    • CI-M6PR antibody
    • CI-MPR antibody
    • CIMPR antibody
    • IGF 2 receptor antibody
    • IGF 2R antibody
    • IGF II receptor antibody
    • IGF-II receptor antibody
    • IGF2 receptor antibody
    • Igf2r antibody
    • Insulin like growth factor 2 receptor antibody
    • Insulin like growth factor II receptor antibody
    • Insulin-like growth factor 2 receptor antibody
    • Insulin-like growth factor II receptor antibody
    • M6P R antibody
    • M6P/IGF2 receptor antibody
    • M6P/IGF2R antibody
    • M6PR antibody
    • mannose 6 phosphate receptor antibody
    • mannose 6 phosphate receptor, cation independent antibody
    • MPR 300 antibody
    • MPR300 antibody
    • MPRI antibody
    • MPRI_HUMAN antibody
    see all

Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker images

  • HeLa cells labelled with anti-mannose-6-phosphate receptor (ab12894). ab12894 was used at a working concentration of 4µg/ml. The arrows indicate the perinuclear location of the mannose-6-phosphate receptor in the late endosomes. In the final panel DAPI is pseudo-coloured red, while the mannose-6-phosphate receptor is in green.

  • ab12894 at 1/100 staining human MCF-7 cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit FITC was used as the secondary.

    See Abreview

References for Anti-M6PR (cation independent) antibody - Lysosome Membrane Marker (ab12894)

This product has been referenced in:
  • Liu Y  et al. Endocytic processing of adeno-associated virus type 8 vectors for transduction of target cells. Gene Ther : (2012). Human . Read more (PubMed: 22622241) »
  • Shen Y  et al. Mutations in PNKD causing paroxysmal dyskinesia alters protein cleavage and stability. Hum Mol Genet 20:2322-32 (2011). ICC/IF ; Human . Read more (PubMed: 21487022) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HELA)
Specification HELA
Fixative Paraformaldehyde
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Apr 30 2012

Thank you for sharing your results through our Abreview system. I am sorry this antibody did not provide the expected results.

I am sorry to hear that you are experiencing difficulties with this product ab12894 in ICC. Often it is possible to...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Heart)
Specification Heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted May 25 2010

Application Immunocytochemistry
Sample African Green Monkey Cultured Cells (kidney)
Specification kidney
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF-7)
Specification MCF-7
Fixative Methanol
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

Submitted Mar 01 2007

Thank you for your enquiry. I am not sure I understand your question, but did notice that the SwissProt database links on the datasheet point to the cation-dependent MPR, not the cation-independent. If this is your question, I am having the databas...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa and SK-N-SH)
Specification HeLa and SK-N-SH
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Oct 12 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"