Anti-M6PR (cation independent) antibody [MEM-238] (ab8093)
Key features and details
- Mouse monoclonal [MEM-238] to M6PR (cation independent)
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-M6PR (cation independent) antibody [MEM-238]
See all M6PR (cation independent) primary antibodies -
Description
Mouse monoclonal [MEM-238] to M6PR (cation independent) -
Host species
Mouse -
Specificity
CD222 antigen (human) -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to Vaccinia virus M6PR (cation independent).
-
Epitope
Recognizes an epitope between domains 2 and 5. -
Positive control
- Flow Cyt (Intra): HeLa cells.
-
General notes
CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Primary antibody notes
CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW]. -
Clonality
Monoclonal -
Clone number
MEM-238 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Predicted molecular weight: 274 kDa.
|
|
Flow Cyt (Intra) |
Use a concentration of 2 - 6 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Predicted molecular weight: 274 kDa. |
Flow Cyt (Intra)
Use a concentration of 2 - 6 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. -
Sequence similarities
Belongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain. -
Domain
Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends. -
Cellular localization
Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. - Information by UniProt
-
Database links
- Entrez Gene: 3482 Human
- Omim: 147280 Human
- SwissProt: P11717 Human
- Unigene: 487062 Human
- Unigene: 673278 Human
-
Alternative names
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
see all
Images
-
All lanes : Anti-M6PR (cation independent) antibody [MEM-238] (ab8093) at 1 µg/ml
Lane 1 : Jurkat whole cell extract, with reducing SDS loading buffer
Lane 2 : K562 whole cell extract, with reducing SDS loading buffer
Lane 3 : Raji whole cell extract, with reducing SDS loading buffer
Lane 4 : HeLa whole cell extract, with reducing SDS loading buffer
Lane 5 : Jurkat whole cell extract, with non-reducing SDS loading buffer
Lane 6 : K562 whole cell extract, with non-reducing SDS loading buffer
Lane 7 : Raji whole cell extract, with non-reducing SDS loading buffer
Lane 8 : HeLa whole cell extract, with non-reducing SDS loading buffer
Secondary
All lanes : IRDye 800CW Goat-anti-Mouse IgG (green)
Predicted band size: 274 kDa
Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, K562, Raji, and HeLa cell lines, mixed and heated (100°C, 5 min) with reducing and non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis.
Nitrocellulose membrane blot was probed with ab8093 followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.
CD222 molecules were detected at ~250 kDa in all analysed cell lines. -
Flow cytometry surface staining analysis of human peripheral whole blood showing separation of human neutrophil granulocytes (red) from lymphocytes black using ab8093 at 2 μg/ml GAM APC.
-
Flow cytometry analysis of human peripheral whole blood stained using ab8093 in 2 μg/ml and GAM APC.
-
Overlay histogram showing HeLa cells stained with ab8093 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8093, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
-
SDS download
-
Datasheet download
References (14)
ab8093 has been referenced in 14 publications.
- Besemer AS et al. Receptor-mediated endocytosis 8 (RME-8)/DNAJC13 is a novel positive modulator of autophagy and stabilizes cellular protein homeostasis. Cell Mol Life Sci 78:645-660 (2021). PubMed: 32322926
- Promchan K & Natarajan V Leucine zipper transcription factor-like 1 binds adaptor protein complex-1 and 2 and participates in trafficking of transferrin receptor 1. PLoS One 15:e0226298 (2020). PubMed: 31895934
- Hirst J et al. Role of the AP-5 adaptor protein complex in late endosome-to-Golgi retrieval. PLoS Biol 16:e2004411 (2018). WB ; Human . PubMed: 29381698
- Stadlmann J et al. Comparative glycoproteomics of stem cells identifies new players in ricin toxicity. Nature 549:538-542 (2017). PubMed: 28959962
- Chen Y et al. Segregation in the Golgi complex precedes export of endolysosomal proteins in distinct transport carriers. J Cell Biol 216:4141-4151 (2017). PubMed: 28978644