Anti-M6PR (cation independent) antibody [MEM-238] (FITC) (ab58841)

Overview

  • Product nameAnti-M6PR (cation independent) antibody [MEM-238] (FITC)
    See all M6PR (cation independent) primary antibodies
  • Description
    Mouse monoclonal [MEM-238] to M6PR (cation independent) (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Recombinant Vaccinia virus encoding Mannose 6 Phosphate Receptor (Cation independent) (CD222).

  • Epitopeab58841 recognizes an epitope between domains 2 and 5 of Mannose 6 Phosphate Receptor (Cation independent) (CD222).
  • Positive control
    • Jurkat whole cell lysate
  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab58841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
WB Use at an assay dependent concentration. Predicted molecular weight: 275 kDa. (non-reducing conditions).
Flow Cyt Use 8.3µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionTransport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
  • Sequence similaritiesBelongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.
  • DomainContains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
  • Cellular localizationLysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • 300 kDa mannose 6 phosphate receptor antibody
    • 300 kDa mannose 6-phosphate receptor antibody
    • Cation independent mannose 6 phosphate receptor antibody
    • Cation-independent mannose-6-phosphate receptor antibody
    • CD222 antibody
    • CD222 antigen antibody
    • CI Man 6 P receptor antibody
    • CI Man-6-P receptor antibody
    • CI MPR antibody
    • CI-M6PR antibody
    • CI-MPR antibody
    • CIMPR antibody
    • IGF 2 receptor antibody
    • IGF 2R antibody
    • IGF II receptor antibody
    • IGF-II receptor antibody
    • IGF2 receptor antibody
    • Igf2r antibody
    • Insulin like growth factor 2 receptor antibody
    • Insulin like growth factor II receptor antibody
    • Insulin-like growth factor 2 receptor antibody
    • Insulin-like growth factor II receptor antibody
    • M6P R antibody
    • M6P/IGF2 receptor antibody
    • M6P/IGF2R antibody
    • M6PR antibody
    • mannose 6 phosphate receptor antibody
    • mannose 6 phosphate receptor, cation independent antibody
    • MPR 300 antibody
    • MPR300 antibody
    • MPRI antibody
    • MPRI_HUMAN antibody
    see all

Anti-M6PR (cation independent) antibody [MEM-238] (FITC) images

  • ab58841 staining Mannose 6 Phosphate Receptor in a Human cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% NP-40 + 1% BSA in PBS and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/50 in 0.5% NP-40 + 1% BSA in PBS) for 1 hour at 25°C.

    See Abreview

  • Lane 1 : Anti-M6PR (cation independent) antibody [MEM-238] (FITC) (ab58841)
    Lane 2 : Mouse IgG1 isotype control

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Jurkat whole cell lysate


    Predicted band size : 275 kDa
  • Overlay histogram showing HeLa cells stained with ab58841 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58841, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-M6PR (cation independent) antibody [MEM-238] (FITC) (ab58841)

This product has been referenced in:
  • Laube F Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression. Anticancer Res 29:1383-8 (2009). Inhibition Assay ; Human . Read more (PubMed: 19414391) »
  • Leksa V  et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). WB, Flow Cyt ; Human . Read more (PubMed: 12189157) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (HEK293, HeLa)
Specification HEK293, HeLa
Permeabilization Yes - 0.5% NP-40, 1%BSA in PBS
Fixative Paraformaldehyde
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Dr. Monica Pons

Verified customer

Submitted Oct 01 2013

Thank you for contacting us. The following two papers include protocols with this clone, MEM-238, which indicate that the antibody recognizes an extracellular epitope. This will also be the case for the FITC conjugate, ab58441. (The unconjugated versio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"