Anti-M6PR (cation independent) antibody [MEM-238] (Phycoerythrin) (ab77135)

Overview

  • Product name
    Anti-M6PR (cation independent) antibody [MEM-238] (Phycoerythrin)
    See all M6PR (cation independent) primary antibodies
  • Description
    Mouse monoclonal [MEM-238] to M6PR (cation independent) (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Recombinant Vaccinia virus encoding CD222.

  • Epitope
    Ab77135 recognizes an epitope between domains 2 and 5 of CD222
  • Positive control
    • Human blood cells

Properties

Applications

Our Abpromise guarantee covers the use of ab77135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20 µl for 106 cells in a suspension or 100 µl of whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
    • Sequence similarities
      Belongs to the MRL1/IGF2R family.
      Contains 1 fibronectin type-II domain.
    • Domain
      Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
    • Cellular localization
      Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • 300 kDa mannose 6 phosphate receptor antibody
      • 300 kDa mannose 6-phosphate receptor antibody
      • Cation independent mannose 6 phosphate receptor antibody
      • Cation-independent mannose-6-phosphate receptor antibody
      • CD222 antibody
      • CD222 antigen antibody
      • CI Man 6 P receptor antibody
      • CI Man-6-P receptor antibody
      • CI MPR antibody
      • CI-M6PR antibody
      • CI-MPR antibody
      • CIMPR antibody
      • IGF 2 receptor antibody
      • IGF 2R antibody
      • IGF II receptor antibody
      • IGF-II receptor antibody
      • IGF2 receptor antibody
      • Igf2r antibody
      • Insulin like growth factor 2 receptor antibody
      • Insulin like growth factor II receptor antibody
      • Insulin-like growth factor 2 receptor antibody
      • Insulin-like growth factor II receptor antibody
      • M6P R antibody
      • M6P/IGF2 receptor antibody
      • M6P/IGF2R antibody
      • M6PR antibody
      • mannose 6 phosphate receptor antibody
      • mannose 6 phosphate receptor, cation independent antibody
      • MPR 300 antibody
      • MPR300 antibody
      • MPRI antibody
      • MPRI_HUMAN antibody
      see all

    References

    This product has been referenced in:
    • Leksa V  et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). Read more (PubMed: 12189157) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for contacting Abcam regarding ab77135.

    Regarding the expiration date for this antibody, when stored at 4oC, it has an expected shelf life of up to 1 year.

    I hope this information is helpful. Please do not hesitate to co...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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