For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Recombinant full length His tagged mCherry purified from E. coli.
Our Abpromise guarantee covers the use of ab125096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 28 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
Immunocytochemical analysis of HEK293 cells labelling mCherry which has been transfected into cells and labelled using ab125096 (with a red secondary). DAPI was used to stain DNA meaning only their neuclei are visualised in blue.
ab125096 staining mCherry in smooth muscle cells in rat bladder by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 10% buffered formalin and blocked with 5000ug/ml BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat polyclonal anti mouse (1/400) was used as the secondary antibody.
ab125096 staining mCherry in Human pancreatic islet tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0Triton X-100 and blocked with BSA + Milk for 1 hour at 25°C. Samples were incubated with primary antibody (1/250) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"