Overview

  • Product nameAnti-mCherry antibody [1C51]
    See all mCherry primary antibodies
  • Description
    Mouse monoclonal [1C51] to mCherry
  • Tested applicationsWB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Immunogen

    Recombinant full length His tagged mCherry purified from E. coli.

  • Positive control
    • HEK293 crude extract transfected with pFin-EF1-mCherry; Hek293 cells transfected with mCherry.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.067% Sodium azide
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone number1C51
  • IsotypeIgG2a
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab125096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 28 kDa.
ICC/IF 1/500.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • RelevancemCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

Anti-mCherry antibody [1C51] images

  • ab125096 staining mCherry in Human pancreatic islet tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0Triton X-100 and blocked with BSA + Milk for 1 hour at 25°C. Samples were incubated with primary antibody (1/250) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab125096, at 1/500 dilution staining mCherry in HEK293 cells transfected with mCherry (visualized in red). Transfected cells are yellow, showing overlap of the mCherry and ab125096. Nontransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue).

References for Anti-mCherry antibody [1C51] (ab125096)

This product has been referenced in:
  • Zhao L  et al. Notch signaling regulates cardiomyocyte proliferation during zebrafish heart regeneration. Proc Natl Acad Sci U S A 111:1403-8 (2014). Read more (PubMed: 24474765) »
  • Boender AJ  et al. Combined use of the canine adenovirus-2 and DREADD-technology to activate specific neural pathways in vivo. PLoS One 9:e95392 (2014). IHC-Fr . Read more (PubMed: 24736748) »

See all 8 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (BT474 Cells)
Specification BT474 Cells
Permeabilization Yes - 1% NP40
Fixative Paraformaldehyde
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Verified customer

Submitted May 06 2014


I can confirm that we have tested this antibody with DsRed and it works well.

Application Immunohistochemistry (Frozen sections)
Blocking step BSA+Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Tissue sections (Pancreatic islet)
Specification Pancreatic islet
Permeabilization Yes - Triton X-100
Fixative Formaldehyde
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Submitted Oct 11 2013

Application IHC - Wholemount
Sample Zebrafish Embryo (To detect exogenous mCherry expression in brain)
Specification To detect exogenous mCherry expression in brain
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Submitted Feb 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (mCherry labeled smooth muscle cells in Bladder)
Specification mCherry labeled smooth muscle cells in Bladder
Fixative 10% Buffered Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: .01M Sodium Citrate
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5000µg/mL · Temperature: 22°C
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Verified customer

Submitted Jan 29 2013

Thank you for your patience. The originator of the antibody anti-mCherry ab125096 has confirmed that it cross-reacts with mTomato. Please let us know if you have any other questions.

Thank you for contacting us. I contacted the laboratory and have some data on two antibodies, the mCHerry antibody ab125096 should only have mild cross-reactivity with tomato and not with tag-RFP or GFP. This antibody as not yet been tested in IP, howe...

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Thank you for contacting us.

1. To our knowledge the ab125096 has not been tested in IHC-Fr yet and we can not guarantee the product to detect the mCherry in IHC-Fr. Regrettably do not offer any other mCherry antibody. However, if the customer...

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Thank you for your enquiry.

I can confirm that this antibody has been tested and is covered by the 6 month guarantee in ICC-IF. As the mcherry is not species related, it should detect the mcherry if you have an mcherry tagged recombinant pro...

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Thank you very much for your reply and for sending this information.

Is the antigen retrieval done in a pressure cooker or on a hotplate? 5 minutes might not be enough time to unmask the epitopes at normal pressure. I would recommend boiling ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"