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Overview

  • Product nameAnti-mCherry antibody [1C51]See all mCherry primary antibodies ...
  • Description
    Mouse monoclonal [1C51] to mCherry
  • Tested applicationsWB, ICC/IF, IHC-P more details
  • Immunogen

    Recombinant full length His tagged mCherry purified from E. coli.

  • Positive controlHEK293 crude extract transfected with pFin-EF1-mCherry; Hek293 cells transfected with mCherry.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.067% Sodium azide
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone number1C51
  • IsotypeIgG2a
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab125096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/2000. Detects a band of approximately 28 kDa.
ICC/IF ICC/IF: 1/500.
IHC-P IHC-P: Use at an assay dependent concentration.

Target

  • RelevancemCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.

Anti-mCherry antibody [1C51] images

  • Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [1C51] (ab125096)
    Immunocytochemistry/ Immunofluorescence - Anti-mCherry antibody [1C51] (ab125096)
    ab125096, at 1/500 dilution staining mCherry in HEK293 cells transfected with mCherry (visualized in red). Transfected cells are yellow, showing overlap of the mCherry and ab125096. Nontransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue).

References for Anti-mCherry antibody [1C51] (ab125096)

This product has been referenced in:
  • Mancio-Silva L  et al. Sir2a regulates rDNA transcription and multiplication rate in the human malaria parasite Plasmodium falciparum. Nat Commun 4:1530 (2013). ICC/IF . Read more (PubMed: 23443558) »

See 1 Publication for this product

Publishing research using ab125096 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab125096 Submit a Question for ab125096

Displaying 1 - 10 of 32 results for Abreviews and Q&A

10
Votes: Highest First

IHC - Wholemount abreview for Anti-mCherry antibody [1C51]

 Poor
Abreviews
Application IHC - Wholemount
Sample Zebrafish Embryo (To detect exogenous mCherry expression in brain)
Specification To detect exogenous mCherry expression in brain
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Verified customer

Submitted Feb 20 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mCherry antibody [1C51]

 Good
Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (mCherry labeled smooth muscle cells in Bladder)
Specification mCherry labeled smooth muscle cells in Bladder
Fixative 10% Buffered Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: .01M Sodium Citrate
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5000&##181;g/mL · Temperature: 22°C
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Verified customer

Submitted Jan 29 2013

Will this antibody cross react with mTomato?

Thank you for your patience. The originator of the antibody anti-mCherry ab125096 has confirmed that it cross-reacts with mTomato. Please let us know if you have any other questions.

Will this antibody cross-react with tag-GFP or GFP?

Thank you for contacting us. I contacted the laboratory and have some data on two antibodies, the mCHerry antibody ab125096 should only have mild cross-reactivity with tomato and not with tag-RFP or GFP. This antibody as not yet been tested in IP, however...

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Dear Abcam Technical Support,

A customer has contacted us with a technical enquiry regarding the following antibodies:

http://www.abcam.com/mcherry-antibody-1c51-ab125096.html (Anti-mCherry antibody [1C51]) and http://www.abcam.com/gfp-...

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Thank you for contacting us.

1. To our knowledge the ab125096 has not been tested in IHC-Fr yet and we can not guarantee the product to detect the mCherry in IHC-Fr. Regrettably do not offer any other mCherry antibody. However, if the customer li...

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Inquiry: Dear, Can I use this antibody to label zebrafish cells after a cytospin? I will have to use a secondary anti-mouse Ab to detect? I would like to visualize the cells by (fluorescence)microscopy. Regards,

Thank you for your enquiry.

I can confirm that this antibody has been tested and is covered by the 6 month guarantee in ICC-IF. As the mcherry is not species related, it should detect the mcherry if you have an mcherry tagged recombinant protei...

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Thanks for helping us with this issue. Below are the answers for your
questions, if you need more information, please let me know.
1) Do you know the lot numbers of these antibodies, or the order or PO
number?

2) What fixativ...

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Thank you very much for your reply and for sending this information.

Is the antigen retrieval done in a pressure cooker or on a hotplate? 5 minutes might not be enough time to unmask the epitopes at normal pressure. I would recommend boiling for...

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Dear tech specialist,
We have encourtered problems with two antibodies Ab9110 and Ab125096.
Please check the attachments for details.
Best,

Thank you very much for contacting us and letting us know about the trouble with these antibodies.

I am sorry to see that the results have been poor. I do have some additional questions so that I better understand the situation:

1) Do ...

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I was just wondering whether your mCherry antibody is the same as that from XXXX, as all the information about it is identical, including the photo, but the price of theirs is €80 cheaper than yours for the same volume.
Best Regards,

Thank you for contacting us.


I can confirm that, though this product does have the same image but this is not a XXXX product.

The price of Abcam products justifies the round of clock technical support and customer service we provide...

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I have a question about your anti-mCherry. I am doing IP with GFP antibody pulldown the GFP-tagged protein and then test another protein with mCherry antibody. I want to know whether your anti-mCherry is specific to mCherry protein? Have you done any expe...

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Thank you for contacting us.


We know that the mCherry antibody does not bind GFP on blots, as we did test for that. The antibody picked up transfected mCherry in Hek293 in a whole cell extract without any problem. We saw a band at the expe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"