Anti-mCherry antibody (ab183628)
Key features and details
- Rabbit polyclonal to mCherry
- Suitable for: ICC/IF, IHC - Wholemount, IP, WB
- Reacts with: Species independent
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-mCherry antibody
See all mCherry primary antibodies -
Description
Rabbit polyclonal to mCherry -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC - Wholemount, IP, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant full length protein corresponding to mCherry.
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Positive control
- IHC-Wm: 2 days-post-fertilization zebrafish embryos . WB: Full length recombinant mCherry. mCherry-tagged protein expressing HEK-293T cell lysate. ICC/IF: mCherry fusion protein-transfected HeLa cells IP: HEK-293T
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
1/100 - 1/1000.
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IHC - Wholemount |
1/100 - 1/500.
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IP | (2) |
1/100 - 1/500.
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IHC - Wholemount
1/100 - 1/500. |
IP
1/100 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa. |
Target
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Relevance
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
Images
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RABV and mGluR2 are internalized into cells and transported together in early and late endosomes.
N2a (Mouse neuroblastoma cells) cells were stained by using the Tyramide Signal Amplification immunofluorescent method. N2a cells infected with ERA-N-mCherry for 20 minutes at 37°C were used to perform immunofluorescence staining for RABV antigen (red), mGluR2 (green), Rab5 or Rab7 (purple), and the cell nuclei (blue). Co-localization of the RABV-mGluR2 complex with Rab5 (E, F).
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Whole mount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryos labeling mCherry-expressing cells with ab183628 at 1/100 dilution.
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All lanes : Anti-mCherry antibody (ab183628) at 1/2000 dilution
Lane 1 : Full length recombinant mCherry at 0.005 µg
Lane 2 : Full length recombinant mCherry at 0.01 µg
Lane 3 : Full length recombinant mCherry at 0.02 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 27 kDa -
All lanes : Anti-mCherry antibody (ab183628) at 1/5000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract
Lane 2 : Whole cell lysate/extract of mCherry-tagged protein expressing HEK-293T cells
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa -
Immunoprecipitation analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract using ab183628 at a 1/3000 dilution.
Lane 1: 20 μg whole cell lysate/extract of Cherry protein-expressing HEK-293T cells.
Lane 2: Control with 2.5 μg of pre-immune rabbit IgG.
Lane 3: Immunoprecipitation of mCherry protein with 2.5 μg of ab183628.7.5% SDS-PAGE. An anti-rabbit IgG (HRP) was used as a secondary reagent.
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Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature and then permeabilized with 0.1% Triton X-100 for 1 minute. mCherry (green) was labeled using ab183628 at a dilution of 1/500.
Red: mCherry fusion protein is expressed in the transfected cell.
Blue: Hoechst 33342 staining. -
Immunofluorescent analysis of mCherry fusion protein-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature and then permeabilized with 0.1% Triton X-100 for 1 minute. mCherry (green) was labeled using ab183628 at a dilution of 1/500.
Red: mCherry fusion protein is expressed in the transfected cell.
Blue: Hoechst 33342 staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (68)
ab183628 has been referenced in 68 publications.
- Brandimarti R et al. The US9-Derived Protein gPTB9TM Modulates APP Processing Without Targeting Secretase Activities. Mol Neurobiol 60:1811-1825 (2023). PubMed: 36576708
- Pannek A et al. Dynamics of intracellular neonatal Fc receptor-ligand interactions in primary macrophages using biophysical fluorescence techniques. Mol Biol Cell 33:ar6 (2022). PubMed: 34731029
- Zhang X et al. Exostosin glycosyltransferase 1 reduces porcine reproductive and respiratory syndrome virus infection through proteasomal degradation of nsp3 and nsp5. J Biol Chem 298:101548 (2022). PubMed: 34971707
- Ferrandiz N et al. Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes. J Cell Biol 221:N/A (2022). PubMed: 35486148
- Saha B et al. Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5α in mitophagy. Cell Rep 39:110797 (2022). PubMed: 35545034