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Anti-mSin3A antibody - ChIP Grade (ab3479)
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Proteins and Peptides
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Western blot - Anti-mSin3A antibody - ChIP Grade (ab3479)
Predicted band size : 145 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mSin3A antibody (ab3479)
ab3479 (2µg/ml) staining mSin3A in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei of epithelial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
mSin3A antibody for WB in Human (3479)
mSin3A antibody for WB in Mouse (3479)
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