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Overview
Product name
Anti-mSin3A antibody - ChIP Grade
See all mSin3A products (4) ...
Description
Rabbit polyclonal to mSin3A - ChIP Grade
Tested applications
CHIPseq, IP, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: MKRRLDDQESDVYAAQQRR, corresponding to amino acids 1-19 of Mouse mSin3A.
MKRRLDDQES DVYAAQQRR
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
CHIPseq: Use at an assay dependent dilution. (PubMed: 21490601)
IP: Use at an assay dependent dilution.
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 150 kDa (predicted molecular weight: 145 kDa).
IHC-P: Use a concentration of 2 µg/ml
Target
Relevance
Mammalian Sin 3 (mSin 3) is a protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin 3A and mSin 3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin 3A and mSin 3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC 1, HDAC 2, RbAp 46, the methyl CpG binding protein MeCP 2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
Cellular localization
Nuclear. Nucleolus. Note=Recruited to the nucleolus by SAP30L.
Alternative names
- DKFZP434K2235 antibody
- FLJ90319 antibody
- Histone deacetylase complex subunit Sin 3a antibody
see all
Database links
- Entrez Gene: 25942 Human
- Entrez Gene: 20466 Mouse
- Omim: 607776 Human
- SwissProt: Q96ST3 Human
- SwissProt: Q60520 Mouse
Anti-mSin3A antibody - ChIP Grade images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mSin3A antibody (ab3479)
ab3479 (2µg/ml) staining mSin3A in human duodenum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclei of epithelial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-mSin3A antibody - ChIP Grade (ab3479)
This product has been referenced in:
- Bantscheff Met al. Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes. Nat Biotechnol : (2011).Read more (PubMed: 21258344) »
- Williams Ket al. TET1 and hydroxymethylcytosine in transcription and DNA methylation fidelity. Nature : (2011). WB, CHIPseq; Mouse.Read more (PubMed: 21490601) »
See all 6 publications for this product
Publishing research using ab3479? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"