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Western blot - mSin3A antibody (ab70727)
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Immunoprecipitation - mSin3A antibody (ab70727)
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Product Name
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mSin3A antibody
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See all mSin3A antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to mSin3A
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Immunogen
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Synthetic peptide corresponding a region between residue 375 and 425 of human mSin3A (NP_056292.1).
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 171 kDa (predicted molecular weight: 145 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysates from HeLa, 293T, and mouse NIH3T3 cells.
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Cellular localization
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Nuclear. Nucleolus. Note=Recruited to the nucleolus by SAP30L.
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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Mammalian Sin 3 (mSin 3) is a protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin 3A and mSin 3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin 3A and mSin 3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC 1, HDAC 2, RbAp 46, the methyl CpG binding protein MeCP 2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
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mSin3A antibody (ab70727) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this mSin3A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for mSin3A antibody images, references, products related to ab70727 and other tools.
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mSin3A antibody images:
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 Western blot - mSin3A antibody (ab70727)
All lanes : mSin3A antibody (ab70727) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from mouse NIH3T3 cells at 50 µg
developed using the ECL technique
Predicted band size : 145 kDa
Observed band size : 145,171 kDa (why is the actual band size different from the predicted?)
Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
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Immunoprecipitation - mSin3A antibody (ab70727)
Detection of Human mSin3A by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70727 at 3 µg/mg lysate. Subsequent Western blot detection of mSin3A was performed using ab70727 at 1 µg/ml. Detected by chemiluminescence with an exposure time of 10 seconds.
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Search PubMed (MEDLINE) for references to mSin3A
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mSin3A antibody - more information
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Customer reviews (feedback) regarding mSin3A antibody |
Customer FAQs regarding mSin3A antibody |
Protocols for mSin3A antibody |
Price and availability of products related to mSin3A antibody |
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mSin3A antibody - related products:
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mSin3A antibody - other tools:
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Contact Abcam with a Technical Enquiry about mSin3A antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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