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Overview

  • Product nameAnti-mTOR antibody (Agarose)See all mTOR primary antibodies ...
  • Description
    Rabbit polyclonal to mTOR (Agarose)
  • ConjugationAgarose
  • Tested applicationsIP more details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (Human)encoded in exons 5 and 6.

  • General notesThere is 100ug of antibody per 200ul of agarose, and the agarose is supplied as a 50% slurry in a total of 400ul volume.

Properties

Applications

Our Abpromise guarantee covers the use of ab19207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: Use at an assay dependent dilution.

Target

  • FunctionKinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • Tissue specificityExpressed in numerous tissues, with highest levels in testis.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications    Autophosphorylated; when part of mTORC1 or mTORC2.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.

    • Target information above from: UniProt accession P42345 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibodyFK506 binding protein 12 rapamycin associated protein 1 antibodyFK506 binding protein 12 rapamycin associated protein 2 antibody
        FK506 binding protein 12 rapamycin complex associated protein 1 antibodyFK506 binding protein12-rapamycin associated protein 1 antibodyFK506 binding protein12-rapamycin associated protein 1 antibodyFK506 binding protein12-rapamycin associated protein 2 antibodyFK506 binding protein12-rapamycin associated protein 2 antibodyFK506-binding protein 12-rapamycin complex-associated protein 1 antibodyFKBP rapamycin associated protein antibodyFKBP12 rapamycin complex associated protein antibodyFKBP12-rapamycin complex-associated protein antibodyFLJ44809 antibodyFRAP antibodyFRAP1 antibodyFRAP1 antibodyFRAP2 antibodyFRAP2 antibodyMammalian target of rapamycin antibodyMechanistic target of rapamycin antibodymTOR antibodyMTOR_HUMAN antibodyOTTHUMP00000001983 antibodyRAFT1 antibodyRAFT1 antibodyRapamycin and FKBP12 target 1 antibodyRapamycin associated protein FRAP2 antibodyRapamycin target protein 1 antibodyRapamycin target protein antibodyRAPT1 antibodyRAPT1 antibodySerine/threonine-protein kinase mTOR antibody
      see all

    References for Anti-mTOR antibody (Agarose) (ab19207)

    This product has been referenced in:
    • Doghman M  et al. Regulation of insulin-like growth factor-mammalian target of rapamycin signaling by microRNA in childhood adrenocortical tumors. Cancer Res 70:4666-75 (2010). Read more (PubMed: 20484036) »
    • Mavrakis M  et al. Depletion of type IA regulatory subunit (RIalpha) of protein kinase A (PKA) in mammalian cells and tissues activates mTOR and causes autophagic deficiency. Hum Mol Genet 15:2962-71 (2006). Read more (PubMed: 16963469) »

    See all 2 Publications for this product

    Publishing research using ab19207 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"