You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-mTOR antibody [Y391] - ChIP Grade (ab32028)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-mTOR antibody [Y391] - ChIP Grade
See all mTOR products (23) ...

Description

Rabbit monoclonal [Y391] to mTOR - ChIP Grade

Tested applications

IHC-P, WB, ICC/IF, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues near the C-term of PI3K/PI4K domain of human mTOR/FRAP was used as an immunogen

Epitope

ab32028 reacts with an epitope located in the C terminal region of mTOR.

Positive control

Hela cell lysate, MCF7 cell lysate, human prostate carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

Y391

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Obesity
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

  • Flow Cytometry-mTOR antibody [Y391] - ChIP Grade(ab32028)Flow Cytometry-mTOR antibody [Y391] - ChIP Grade(ab32028) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - mTOR antibody [Y391] (ab32028)Immunohistochemistry (Paraffin-embedded sections) - mTOR antibody [Y391] (ab32028) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence-mTOR antibody [Y391](ab32028)Immunocytochemistry/ Immunofluorescence-mTOR antibody [Y391](ab32028) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Tissue specificity

Expressed in numerous tissues, with highest levels in testis.

Sequence similarities

Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain.

Post-translational
modifications

Autophosphorylated; when part of mTORC1 or mTORC2.

Cellular localization

Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.

Target information above from: UniProt accessionP42345 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
  • FK506 binding protein 12 rapamycin associated protein 1 antibody
  • FK506 binding protein 12 rapamycin associated protein 2 antibody
  • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
  • FK506 binding protein12-rapamycin associated protein 1 antibody
  • FK506 binding protein12-rapamycin associated protein 2 antibody
  • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
  • FKBP rapamycin associated protein antibody
  • FKBP12 rapamycin complex associated protein antibody
  • FKBP12-rapamycin complex-associated protein antibody
  • FLJ44809 antibody
  • FRAP antibody
  • FRAP1 antibody
  • FRAP2 antibody
  • Mammalian target of rapamycin antibody
  • Mechanistic target of rapamycin antibody
  • mTOR antibody
  • MTOR_HUMAN antibody
  • OTTHUMP00000001983 antibody
  • RAFT1 antibody
  • Rapamycin and FKBP12 target 1 antibody
  • Rapamycin associated protein FRAP2 antibody
  • Rapamycin target protein 1 antibody
  • Rapamycin target protein antibody
  • RAPT1 antibody
  • Serine/threonine-protein kinase mTOR antibody
see all

Database links

see all

Anti-mTOR antibody [Y391] - ChIP Grade images:

  Flow Cytometry-mTOR antibody [Y391] - ChIP Grade(ab32028)

Flow Cytometry-mTOR antibody [Y391] - ChIP Grade(ab32028)

Overlay histogram showing HeLa cells stained with ab32028 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32028, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

  Immunohistochemistry (Paraffin-embedded sections) - mTOR antibody [Y391] (ab32028)

Immunohistochemistry (Paraffin-embedded sections) - mTOR antibody [Y391] (ab32028)

Immunohistochemical analysis of mTOR expression in paraffin-embedded human prostate carcinoma, using 1/250 ab32028.

  Immunocytochemistry/ Immunofluorescence-mTOR antibody [Y391](ab32028)

Immunocytochemistry/ Immunofluorescence-mTOR antibody [Y391](ab32028)

ICC/IF image of ab32028 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32028, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Western blot - mTOR antibody [Y391] (ab32028)

Western blot - mTOR antibody [Y391] (ab32028)

All lanes : Anti-mTOR antibody [Y391] - ChIP Grade (ab32028) at 1/2000 dilution

Lane 1 : Hela cell lysate
Lane 2 : MCF-7


Predicted band size : 289 kDa
Observed band size : >250 kDa (why is the actual band size different from the predicted?)

References for Anti-mTOR antibody [Y391] - ChIP Grade (ab32028)

This product has been referenced in:

  • Fleming GF  et al. Phase II trial of temsirolimus in patients with metastatic breast cancer. Breast Cancer Res Treat : (2012).Read more (PubMed: 22245973) »
  • Gant JC  et al. Disrupting function of FK506-binding protein 1b/12.6 induces the Ca²+-dysregulation aging phenotype in hippocampal neurons. J Neurosci 31:1693-703 (2011). Rat.Read more (PubMed: 21289178) »

See all 4 publications for this product

Publishing research using ab32028? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"