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- Secondary antibodies
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Product name
Anti-mTOR antibody
See all mTOR products (23) ...
Description
Rabbit polyclonal to mTOR
Specificity
ab34758 recognises both phosphorylated and non-phosphorylated forms of mTOR at S2448 from human derived tissues and cells.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog, Zebrafish
Immunogen
Synthetic peptide: rsrtrtdsysagqsveil, corresponding to amino acids 2440-2457 of Human mTOR
rsrtrtdsysagqsveil
Positive control
HEK293 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Obesity
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Western blot - mTOR antibody (ab34758)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/8000 - 1/32000.
WB: 1/500 - 1/3000. Detects a band of approximately 250 kDa (predicted molecular weight: 289 kDa).
Target
Function
Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Tissue specificity
Expressed in numerous tissues, with highest levels in testis.
Sequence similarities
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain.
Post-translational
modifications
Autophosphorylated; when part of mTORC1 or mTORC2.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
Target information above from: UniProt accessionP42345
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
- FK506 binding protein 12 rapamycin associated protein 1 antibody
- FK506 binding protein 12 rapamycin associated protein 2 antibody
see all
Database links
- Entrez Gene: 478232 Dog
- Entrez Gene: 2475 Human
- Entrez Gene: 56717 Mouse
- Entrez Gene: 56718 Rat
- Omim: 601231 Human
- SwissProt: P42345 Human
- SwissProt: Q9JLN9 Mouse
- SwissProt: P42346 Rat
see all
Anti-mTOR antibody images:
Western blot - mTOR antibody (ab34758)
Anti-mTOR antibody (ab34758) at 1/650 dilution + 30 µg of HEK293 cell lysate
Secondary
IRDye™800 conjugated Goat anti-Rabbit IgG at 1/10000 dilution
Predicted band size : 289 kDa
Observed band size : 245 kDa (why is the actual band size different from the predicted?)
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
References for Anti-mTOR antibody (ab34758)
ab34758 has not yet been referenced specifically in any publications.
Publishing research using ab34758? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"