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Overview

  • Product nameAnti-mTOR antibodySee all mTOR primary antibodies ...
  • Description
    Rabbit polyclonal to mTOR
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Fruit fly (Drosophila melanogaster)
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 2150 - 2250 of Human mTOR.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab45988.)

  • Positive controlThis antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; HEK293; HepG2 (data not shown for HepG2)

Properties

Applications

Our Abpromise guarantee covers the use of ab45989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 289 kDa).Can be blocked with mTOR peptide (ab45988).

Target

  • FunctionKinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • Tissue specificityExpressed in numerous tissues, with highest levels in testis.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications    Autophosphorylated; when part of mTORC1 or mTORC2.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.

    • Target information above from: UniProt accession P42345 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibodyFK506 binding protein 12 rapamycin associated protein 1 antibodyFK506 binding protein 12 rapamycin associated protein 2 antibody
      FK506 binding protein 12 rapamycin complex associated protein 1 antibodyFK506 binding protein12-rapamycin associated protein 1 antibodyFK506 binding protein12-rapamycin associated protein 1 antibodyFK506 binding protein12-rapamycin associated protein 2 antibodyFK506 binding protein12-rapamycin associated protein 2 antibodyFK506-binding protein 12-rapamycin complex-associated protein 1 antibodyFKBP rapamycin associated protein antibodyFKBP12 rapamycin complex associated protein antibodyFKBP12-rapamycin complex-associated protein antibodyFLJ44809 antibodyFRAP antibodyFRAP1 antibodyFRAP2 antibodyMammalian target of rapamycin antibodyMechanistic target of rapamycin antibodyMTOR antibodyMTOR_HUMAN antibodyOTTHUMP00000001983 antibodyRAFT1 antibodyRapamycin and FKBP12 target 1 antibodyRapamycin associated protein FRAP2 antibodyRapamycin target protein 1 antibodyRapamycin target protein antibodyRAPT1 antibodySerine/threonine-protein kinase mTOR antibody
    see all

Anti-mTOR antibody images

  • Western blot - Anti-mTOR antibody (ab45989)
    Western blot - Anti-mTOR antibody (ab45989)
    All lanes : Anti-mTOR antibody (ab45989) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 289 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  • Western blot - mTOR antibody (ab45989)
    Western blot - mTOR antibody (ab45989)
    Anti-mTOR antibody (ab45989) at 1 µg/ml + Human HEK293 whole cell lysate at 12.5 µg

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/16000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 289 kDa
    Observed band size : 289 kDa
    Additional bands at : 180 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-mTOR antibody (ab45989)

ab45989 has not yet been referenced specifically in any publications.

Publishing research using ab45989 ? Please let us know so that we can cite the reference in this datasheet

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Western blot abreview for Anti-mTOR antibody

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 12.5 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jun 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"