Overview

  • Product nameAnti-mTOR antibodySee all mTOR primary antibodies ...
  • Description
    Rabbit polyclonal to mTOR
  • Specificityab51089 detects endogenous levels of total mTOR protein.
  • Tested applicationsIHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthesized non-phosphopeptide derived from human mTOR around the phosphorylation site of serine 2448 (T-D-SP-Y-S).

  • Positive control
    • Human brain tissue and K562 lysates IF/ICC: HeLa cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab51089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 289 kDa.
ELISA 1/40000.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionKinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • Tissue specificityExpressed in numerous tissues, with highest levels in testis.
  • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications
    Autophosphorylated; when part of mTORC1 or mTORC2.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
    • FK506 binding protein 12 rapamycin associated protein 1 antibody
    • FK506 binding protein 12 rapamycin associated protein 2 antibody
    • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
    • FK506 binding protein12-rapamycin associated protein 1 antibody
    • FK506 binding protein12-rapamycin associated protein 2 antibody
    • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
    • FKBP rapamycin associated protein antibody
    • FKBP12 rapamycin complex associated protein antibody
    • FKBP12-rapamycin complex-associated protein antibody
    • FLJ44809 antibody
    • FRAP antibody
    • FRAP1 antibody
    • FRAP2 antibody
    • Mammalian target of rapamycin antibody
    • Mechanistic target of rapamycin antibody
    • mTOR antibody
    • MTOR_HUMAN antibody
    • OTTHUMP00000001983 antibody
    • RAFT1 antibody
    • Rapamycin and FKBP12 target 1 antibody
    • Rapamycin associated protein FRAP2 antibody
    • Rapamycin target protein 1 antibody
    • Rapamycin target protein antibody
    • RAPT1 antibody
    • Serine/threonine-protein kinase mTOR antibody
    see all

Anti-mTOR antibody images

  • ICC/IF image of ab51089 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51089, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-mTOR antibody (ab51089) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : K562 cell extract treated with the immunizing peptide


    Predicted band size : 289 kDa
  • ab51089 staining mTOR in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunizing (blocking) peptide.ab51089 staining mTOR in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunizing (blocking) peptide.

References for Anti-mTOR antibody (ab51089)

This product has been referenced in:
  • Xie L  et al. The frontal cortex IGF system is down regulated in the term, intrauterine growth restricted fetal baboon. Growth Horm IGF Res 23:187-92 (2013). Baboon . Read more (PubMed: 23911858) »
  • Cooper A  et al. Ewing tumors that do not overexpress BMI-1 are a distinct molecular subclass with variant biology: a report from the Children's Oncology Group. Clin Cancer Res 17:56-66 (2011). IHC-P ; Human . Read more (PubMed: 21047978) »

See all 3 Publications for this product

Product Wall

Thank you for your patience.
I have heard back regarding ab51089, ab59299, and ab59350.
These 3 antibodies are predicted to react with rat based on sequence homology but have not been tested yet with samples from this species.
I apologiz...

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Thank you for your reply.
The following mTOR antibodies have been tested in rat as well as IHC-P:
ab32028, ab51089, ab59299, and ab59350.
The antibody ab32028 has been tested in the lab on IHC-P. I am not certain that rat tissue was used...

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In which case, if you are happy with it I will send ab2732 free of charge for you to try. This is a rabbit polyclonal to human mTOR and has been used previously in paraffin embedded sections for IHC. Heat mediated antigen retrieval using citric acid bu...

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Thank you for getting back to me. Unfortunately we no longer have an alternative lot to the one used by you in stock. I can send you an alternative vial (prepared and purified at a different time to the one used by you) or an alternative antibody such ...

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Thank you for contacting us. I'm very sorry for the delay but we have no record of your initial contact regarding ab51089. If this ever happens again please do not hesitate to contact us straight away (by phone or email), we aim to answer all our corre...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"