Anti-mTOR antibody (ab51089)
- Product nameAnti-mTOR antibodySee all mTOR primary antibodies ...
- DescriptionRabbit polyclonal to mTOR
- Specificityab51089 detects endogenous levels of total mTOR protein.
- Tested applicationsIHC-P, WB, ELISA, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
A synthesized non-phosphopeptide derived from human mTOR around the phosphorylation site of serine 2448 (T-D-SP-Y-S).
- Positive control
- Human brain tissue and K562 lysates IF/ICC: HeLa cell line
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesab51089 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Clonality Polyclonal
- Epigenetics and Nuclear Signaling
- DNA / RNA
- DNA Damage & Repair
- DNA Damage Response
- DNA Damage Recognition
Our Abpromise guarantee covers the use of ab51089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use at an assay dependent concentration.|
|WB||WB: 1/500 - 1/1000. Predicted molecular weight: 289 kDa.|
|ICC/IF||ICC/IF: Use a concentration of 1 µg/ml.|
- FunctionKinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
- Tissue specificityExpressed in numerous tissues, with highest levels in testis.
- Sequence similaritiesBelongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain.
modificationsAutophosphorylated; when part of mTORC1 or mTORC2.
- Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
- dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
- FK506 binding protein 12 rapamycin associated protein 1 antibody
- FK506 binding protein 12 rapamycin associated protein 2 antibody
- FK506 binding protein 12 rapamycin complex associated protein 1 antibody
- FK506 binding protein12-rapamycin associated protein 1 antibody
- FK506 binding protein12-rapamycin associated protein 2 antibody
- FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
- FKBP rapamycin associated protein antibody
- FKBP12 rapamycin complex associated protein antibody
- FKBP12-rapamycin complex-associated protein antibody
- FLJ44809 antibody
- FRAP antibody
- FRAP1 antibody
- FRAP2 antibody
- Mammalian target of rapamycin antibody
- Mechanistic target of rapamycin antibody
- mTOR antibody
- MTOR_HUMAN antibody
- OTTHUMP00000001983 antibody
- RAFT1 antibody
- Rapamycin and FKBP12 target 1 antibody
- Rapamycin associated protein FRAP2 antibody
- Rapamycin target protein 1 antibody
- Rapamycin target protein antibody
- RAPT1 antibody
- Serine/threonine-protein kinase mTOR antibody
Anti-mTOR antibody images
ICC/IF image of ab51089 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51089, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-mTOR antibody (ab51089) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : K562 cell extract treated with the immunizing peptide
Predicted band size : 289 kDa
ab51089 staining mTOR in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunizing (blocking) peptide.ab51089 staining mTOR in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunizing (blocking) peptide.
References for Anti-mTOR antibody (ab51089)
This product has been referenced in:
- Xie L et al. The frontal cortex IGF system is down regulated in the term, intrauterine growth restricted fetal baboon. Growth Horm IGF Res 23:187-92 (2013). Baboon . Read more (PubMed: 23911858) »
- Cooper A et al. Ewing tumors that do not overexpress BMI-1 are a distinct molecular subclass with variant biology: a report from the Children's Oncology Group. Clin Cancer Res 17:56-66 (2011). IHC-P ; Human . Read more (PubMed: 21047978) »