Anti-mTOR antibody (ab59350)

Overview

• Product nameAnti-mTOR antibodySee all mTOR primary antibodies ...
• Description
  Rabbit polyclonal to mTOR
• Specificityab59350 detects endogenous levels of total mTOR protein
• Tested applicationsIHC-P, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthesized phosphopeptide derived from human mTOR around the phosphorylation site of serine 2481 (I-H-S^P-F-I).

• Positive controlHuman brain tissue

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺),, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity-purified from The antibody was affinity-purified fromr abbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Obesity

  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Other

  • Cardiovascular
  • Heart
  • Cardiogenesis
  • Transcription factors/regulators

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • DNA Damage Recognition

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Other

Applications

Target

• FunctionKinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
• Tissue specificityExpressed in numerous tissues, with highest levels in testis.
• Sequence similaritiesBelongs to the PI3/PI4-kinase family.
  Contains 1 FAT domain.
  Contains 1 FATC domain.
  Contains 7 HEAT repeats.
  Contains 1 PI3K/PI4K domain.
• Post-translational
  modificationsAutophosphorylated; when part of mTORC1 or mTORC2.
• Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.

Target information above from: UniProt accession P42345 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2475 Human
  • Entrez Gene: 56717 Mouse
  • Entrez Gene: 56718 Rat
  • Omim: 601231 Human
  • SwissProt: P42345 Human
  • SwissProt: Q9JLN9 Mouse
  • SwissProt: P42346 Rat
  • Unigene: 338207 Human

  • Unigene: 21158 Mouse
  • Unigene: 11008 Rat

  see all

• Alternative names
  dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibodyFK506 binding protein 12 rapamycin associated protein 1 antibodyFK506 binding protein 12 rapamycin associated protein 2 antibody

  FK506 binding protein 12 rapamycin complex associated protein 1 antibodyFK506 binding protein12-rapamycin associated protein 1 antibodyFK506 binding protein12-rapamycin associated protein 2 antibodyFK506-binding protein 12-rapamycin complex-associated protein 1 antibodyFKBP rapamycin associated protein antibodyFKBP12 rapamycin complex associated protein antibodyFKBP12-rapamycin complex-associated protein antibodyFLJ44809 antibodyFRAP antibodyFRAP1 antibodyFRAP2 antibodyMammalian target of rapamycin antibodyMechanistic target of rapamycin antibodymTOR antibodyMTOR_HUMAN antibodyOTTHUMP00000001983 antibodyRAFT1 antibodyRapamycin and FKBP12 target 1 antibodyRapamycin associated protein FRAP2 antibodyRapamycin target protein 1 antibodyRapamycin target protein antibodyRAPT1 antibodySerine/threonine-protein kinase mTOR antibody

  see all

Anti-mTOR antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - mTOR antibody (ab59350)
  ab59350, at a 1/50-1/100 dilution, staining human mTOR in brain with (+) or without (-) immunising peptide, using Immunohistochemistry, Paraffin embedded tissue