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Anti-mTOR (phospho S2448) antibody (ab1093)

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Overview

Product name

Anti-mTOR (phospho S2448) antibody
See all mTOR products (23) ...

Description

Rabbit polyclonal to mTOR (phospho S2448)

Specificity

Specific for phospho mTOR, giving a band at approx 250 kDa. This product has been assayed by ELISA against 0.1 ug of the immunizing peptide(not tested against the endogenous protein).

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: KRSRTRTDpSYSAGQSVEI, corresponding to C terminal amino acids 2440-2463 of Human mTOR.

KRSRTRTDpSYSAGQSVEI

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

serum starved 293T cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% Sodium Azide, pH 7.2

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Obesity
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

  • Western blot - mTOR (phospho S2448) antibody (ab1093)Western blot - mTOR (phospho S2448) antibody (ab1093) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mTOR (phospho S2448) antibody (ab1093)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mTOR (phospho S2448) antibody (ab1093) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000. Detects a ba...Read more →

    WB: 1/1000. Detects a band of approximately 250 kDa (predicted molecular weight: 288 kDa).

  • ShowHide

    ELISA

     ELISA: 1/6000 - 1/29000....Read more →

    ELISA: 1/6000 - 1/29000. Assayed aginst 0.1 µg of the immunizing peptide and has not been tested against the endogenous protein.

Target

Function

Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Tissue specificity

Expressed in numerous tissues, with highest levels in testis.

Sequence similarities

Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain.

Post-translational
modifications

Autophosphorylated; when part of mTORC1 or mTORC2.

Cellular localization

Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.

Target information above from: UniProt accessionP42345 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
  • FK506 binding protein 12 rapamycin associated protein 1 antibody
  • FK506 binding protein 12 rapamycin associated protein 2 antibody
  • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
  • FK506 binding protein12-rapamycin associated protein 1 antibody
  • FK506 binding protein12-rapamycin associated protein 2 antibody
  • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
  • FKBP rapamycin associated protein antibody
  • FKBP12 rapamycin complex associated protein antibody
  • FKBP12-rapamycin complex-associated protein antibody
  • FLJ44809 antibody
  • FRAP antibody
  • FRAP1 antibody
  • FRAP2 antibody
  • Mammalian target of rapamycin antibody
  • Mechanistic target of rapamycin antibody
  • mTOR antibody
  • MTOR_HUMAN antibody
  • OTTHUMP00000001983 antibody
  • RAFT1 antibody
  • Rapamycin and FKBP12 target 1 antibody
  • Rapamycin associated protein FRAP2 antibody
  • Rapamycin target protein 1 antibody
  • Rapamycin target protein antibody
  • RAPT1 antibody
  • Serine/threonine-protein kinase mTOR antibody
see all

Database links

Anti-mTOR (phospho S2448) antibody images:

  Western blot - mTOR (phospho S2448) antibody (ab1093)

Western blot - mTOR (phospho S2448) antibody (ab1093)



Predicted band size : 288 kDa


Rabbit polyclonal to phospho mTOR (Ser 2448) at 1/1000 on 293T cell extract shows a band at approx 250 kDa. Cells were serum starved for 24 hours:
Lane 1 : control
Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min

Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mTOR (phospho S2448) antibody (ab1093)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - mTOR (phospho S2448) antibody (ab1093)

ab1093 at 5 µg/ml staining proximal convulted tubules of the kidney.  The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

References for Anti-mTOR (phospho S2448) antibody (ab1093)

This product has been referenced in:

  • Gibbs BF  et al. Effects of Stem Cell Factor on Hypoxia-Inducible Factor 1 Alpha Accumulation in Human Acute Myeloid Leukaemia and LAD2 Mast Cells. PLoS One 6:e22502 (2011). WB; Human.Read more (PubMed: 21799876) »

See 1 publication for this product

Publishing research using ab1093? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"