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Synthetic peptide: KRSRTRTDpSYSAGQSVEI, corresponding to C terminal amino acids 2440-2463 of Human mTOR.
Our Abpromise guarantee covers the use of ab1093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||1/1000. Detects a band of approximately 250 kDa (predicted molecular weight: 288 kDa).|
|ELISA||1/6000 - 1/29000. Assayed aginst 0.1 µg of the immunizing peptide and has not been tested against the endogenous protein.|
Rabbit polyclonal to phospho mTOR (Ser 2448) at 1/1000 on 293T cell extract shows a band at approx 250 kDa. Cells were serum starved for 24 hours:
Lane 1 : control
Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min
Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada
ab1093 at 5 µg/ml staining proximal convulted tubules of the kidney. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
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