The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. This antibody gave a positive result in ELISA against the immunizing peptide (ab46195). It gave a negative result in ELISA against the non-modified equivalent peptide (ab61979). This indicates that it is specific for the modified peptide.
See figure below.
FunctionKinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Tissue specificityExpressed in numerous tissues, with highest levels in testis.
Post-translational modificationsAutophosphorylated; when part of mTORC1 or mTORC2.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
FK506 binding protein 12 rapamycin associated protein 1 antibody
FK506 binding protein 12 rapamycin associated protein 2 antibody
FK506 binding protein 12 rapamycin complex associated protein 1 antibody
FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
FKBP rapamycin associated protein antibody
FKBP12 rapamycin complex associated protein antibody
FKBP12-rapamycin complex-associated protein 1 antibody
FKBP12-rapamycin complex-associated protein antibody
Mammalian target of rapamycin antibody
Mechanistic target of rapamycin antibody
Rapamycin and FKBP12 target 1 antibody
Rapamycin associated protein FRAP2 antibody
Rapamycin target protein 1 antibody
Rapamycin target protein antibody
Serine/threonine-protein kinase mTOR antibody
Anti-mTOR (phospho S2481) antibody images
ELISA - mTOR (phospho S2481) antibody (ab45996)
This antibody gave a positive result in ELISA against the immunizing peptide (Orange line, ab46195). It gave a negative result in ELISA against the non-modified equivalent peptide (Yellow line, ab61979). This indicates that it is specific for the modified peptide.
ICC/IF image of ab45996 stained Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45996, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, HeLa and MCF7 cells fixed in 4% PFA at 1ug/ml and HeLa, Hek293, HepG2 and MCF7 cells fixed in 100% methanol at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
References for Anti-mTOR (phospho S2481) antibody (ab45996)
This product has been referenced in:
Vazquez-Martin A et al. Ser2481-autophosphorylated mTOR colocalizes with chromosomal passenger proteins during mammalian cell cytokinesis. Cell Cycle11:4211-21 (2012).
Read more (PubMed: 23095638) »
Vazquez-Martin A et al. Raptor, a positive regulatory subunit of mTOR complex 1, is a novel phosphoprotein of the rDNA transcription machinery in nucleoli and chromosomal nucleolus organizer regions (NORs). Cell Cycle10:3140-52 (2011).
Read more (PubMed: 21900751) »