Overview

  • Product nameAnti-MACF1 antibody
  • Description
    Rabbit polyclonal to MACF1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 1-50 of Mouse MACF1.

  • Positive control
    • Mouse kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: Proprietary component, Whole serum
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG

Applications

Our Abpromise guarantee covers the use of ab117418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 832 kDa.
ELISA 1/10000.

Target

  • RelevanceMicrotubule-actin crosslinking factor 1 (MACF1/ACF7) belongs to spectraplakin family of proteins which also includes mammalian Bpag1/dystonin, Drosophila Short Stop (Shot) and Caenorhabditis elegans Vab-10. These are large actin-microtubule linker molecules that are essential for various biological processes such as neuronal degeneration, wound healing, gastrulation. MACF1, located on mouse chromosome 4D2.2, was found to play an important role during neurogenesis- it was shown that MACF1 regulates the organization of neuronal microtubules which is essential for axon extension. Furthermore, MACF1 is required in controlling focal adhesion assembly and dynamics. MACF1 behaves as an actin-regulated ATPase and it has the ability to target microtubules that track along F-actin to focal adhesions. MACF1 was also implicated in regulating filopodia formation.
  • Cellular localizationCytoplasmic, microtubule. Plasma membrane. Golgi apparatus.
  • Database links
  • Alternative names
    • ABP620 antibody
    • Acf7 antibody
    • Aclp7 antibody
    • Actin cross linking family 7 antibody
    • MACF antibody
    • Microtubule actin crosslinking factor 1 antibody
    • mKIAA0465 antibody
    • R74989 antibody
    • rabeculin alpha antibody
    see all

Anti-MACF1 antibody images

  • ab117418 at 1/500 dilution staining MACF1 in Mouse kidney tissue by Immunofluorescence.

References for Anti-MACF1 antibody (ab117418)

ab117418 has not yet been referenced specifically in any publications.

Product Wall

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The first thing I recommend checking is whether the transfer worked. You can do this with a Ponceau Red stain, before you incubate with the antibody. The protei...

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