Anti-Macro H2A.2 antibody (ab102126)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Macro H2A.2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 197-340 of Human Macro H2A.2 (AAH16172).

  • Positive control
    • A431 whole cell lysate, TOV-21G xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, PBS, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)

Target

  • Function
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
  • Sequence similarities
    Contains 1 histone H2A domain.
    Contains 1 Macro domain.
  • Cellular localization
    Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Core histone macro H2A2.2 antibody
    • Core histone macro-H2A.2 antibody
    • Core histone macroH2A2.2 antibody
    • H2A histone family member Y2 antibody
    • H2AFY2 antibody
    • H2AW_HUMAN antibody
    • Histone macroH2A2 antibody
    • Macro H2A2 antibody
    • MacroH2A.2 antibody
    • MacroH2A2 antibody
    • mH2A2 antibody
    see all

Anti-Macro H2A.2 antibody images

  • Anti-Macro H2A.2 antibody (ab102126) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 40 kDa
  • Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ab102126 at 1/100 dilution.

References for Anti-Macro H2A.2 antibody (ab102126)

ab102126 has not yet been referenced specifically in any publications.

Product Wall

Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Brain nuclei)
Negative control
IgG
Specification
Brain nuclei
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.5% Fromaldehyde
Positive control
H3
Username

Abcam user community

Verified customer

Submitted Feb 10 2017

Thank you very much for your reply.

All of the information we have for each of our products is listed on the online datasheet and these are updated as soon as we receive any new information. So once we know the identity of the band, you will...

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Thank you very much for your enquiry.

Please accept my apologies for the long delay of my reply. I have been in contact with the source of this antibody regarding the additional band between 72 and 55 kDa. We suspect that this band may be du...

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Application
ChIP
Sample
Mouse Cell lysate - whole cell (ES)
Specification
ES
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
No
Negative control
macroH2a2-/- ES cells
IgG ab
Username

Abcam user community

Verified customer

Submitted Nov 09 2011

I am very pleased to hear you would like to accept our offer and test ab4173 & ab102126 in mouse samples. This code will be worth the price you pay for ab4173 & ab102126 and can be used with any single future primary antibody purchase before the expira...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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